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Literature summary for 1.6.1.2 extracted from
- The membrane-peripheral subunits of transhydrogenase from Entamoeba histolytica are functional only when dimerized (2002), J. Biol. Chem., 277, 26163-26170.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| domains dIII and dI expressed in Escherichia coli, recombinant protein lacks the membrane spanning dII domain | Entamoeba histolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Entamoeba histolytica | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
dIII fragement, SDS-PAGE | Entamoeba histolytica |
| 40000 | - |
dI fragement, SDS-PAGE | Entamoeba histolytica |
| 155000 | - |
gel filtration of a mixture of the two domains | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + NADP+ | Entamoeba histolytica | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, important as source of NADPH for biosynthesis and glutathione reduction | NAD+ + NADPH | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant proteins | Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 25% glycerol | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + NADP+ | - |
Entamoeba histolytica | NAD+ + NADPH | - |
r | |
| NADH + NADP+ | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, important as source of NADPH for biosynthesis and glutathione reduction | Entamoeba histolytica | NAD+ + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * (40000 + 28000), SDS-PAGE, domains dIII and dI form active dimers | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| proton-translocating transhydrogenase | - |
Entamoeba histolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)+ | - |
Entamoeba histolytica | |
| NAD(P)H | - |
Entamoeba histolytica | |
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Release 2023.1 (January 2023)
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