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Literature summary for 1.6.1.2 extracted from

  • Diggle, C.; Bizouarn, T.; Cotton, N.P.; Jackson, J.B.
    Properties of the purified, recombinant, NADP(H)-binding domain III of the proton-translocating nicotinamide nucleotide transhydrogenase from Rhodospirillum rubrum (1996), Eur. J. Biochem., 241, 162-170.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of domains I and III in Escherichia coli Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant domains I and III Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + oxidized 3-acetylpyridine adenine dinucleotide
-
Rhodospirillum rubrum NAD+ + reduced 3-acetylpyridine adenine dinucleotide
-
r
NADPH + oxidized 3-acetylpyridine adenine dinucleotide catalyzed by a mixture of purified recombinant domains I and III Rhodospirillum rubrum NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
oxidized 3-acetylpyridine adenine dinucleotide kcat for recombinant domain III in the presence of saturating amounts of domain I, reduction of acetylpyridine adenine dinucleotide by NADPH Rhodospirillum rubrum
1.44
-
oxidized 3-acetylpyridine adenine dinucleotide kcat for recombinant domain I in the presence of saturating amounts of domain III, reduction of acetylpyridine adenine dinucleotide by NADPH Rhodospirillum rubrum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhodospirillum rubrum
NADH
-
Rhodospirillum rubrum
NADP+
-
Rhodospirillum rubrum
NADPH
-
Rhodospirillum rubrum