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Literature summary for 1.6.1.1 extracted from

  • Collins, P.A.; Knowles, C.J.
    Transhydrogenase activity in the marine bacterium Beneckea natriegens (1977), Biochim. Biophys. Acta, 480, 77-82.
    View publication on PubMed
Search for more literature for 1.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP
-
 Vibrio natriegens
5'-AMP 5 mM, 92% inhibition of NADP+ reduction, complete inhibition of NAD+ reduction Vibrio natriegens
ADP 5 mM, 94% inhibition of NADP+ reduction, complete inhibition of NAD+ reduction Vibrio natriegens
arsenate complete inhibition of activity in either direction Vibrio natriegens
ATP 5 mM, 81% inhibition of NADP+ reduction, complete inhibition of NAD+ reduction Vibrio natriegens
CTP 5 mM, 86% inhibition of NADP+ reduction Vibrio natriegens
diphosphate 5 mM, 91% inhibition of NADP+ reduction Vibrio natriegens
GTP 5 mM, 89% inhibition of NADP+ reduction Vibrio natriegens
phosphate 5 mM, complete inhibition of activity in either direction Vibrio natriegens
phosphoenolpyruvate 87% inhibition of NADP+ reduction Vibrio natriegens
pyridoxal 5'-phosphate 5 mM, 91% inhibition of NADP+ reduction Vibrio natriegens
TTP 2 mM, 71% inhibition of NADP+ reduction Vibrio natriegens

Organism

Organism UniProt Comment Textmining
Vibrio natriegens
-
 formerly Pseudomonas natriegens
-

Purification (Commentary)

Purification (Comment) Organism
partial Vibrio natriegens

Storage Stability

Storage Stability Organism
-15°C, 50 mM Tris-HCl buffer, pH 7.5, no loss of activity in 12 weeks Vibrio natriegens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADP+ + NADH reduction of NADP+ is preferred Vibrio natriegens NADPH + NAD+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 NADH formation Vibrio natriegens
9.6
-
 NADPH formation Vibrio natriegens