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Literature summary for 1.5.99.B2 extracted from
- Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems (2013), J. Chromatogr. B, 929, 11-17 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the enzyme in Escherichia coli strain BL21 (DE3) plysS inclusion bodies | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme via refolding, reconstitution and two-phase partitioning in an optimized aqueous two-phase system, containing 14% w/w PEG-1000 and 12% w/w Na2CO3 at pH 8.0, from Escherichia coli strain BL21 (DE3) plysS inclusion bodies in one step, method optimization and evaluation, overview | Pseudomonas fluorescens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant enzyme via refolding, reconstitution and two-phase partitioning in an optimized aqueous two-phase system, containing 14% w/w PEG-1000 and 12% w/w Na2CO3 at pH 8.0, from Escherichia coli strain BL21 (DE3) plysS inclusion bodies in one step | Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 600 | - |
purified recombinant enzyme in an optimized aqueous two-phase system containing 14% w/w PEG-1000 and 12% w/w Na2CO3, pH 8.0, 37°C | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-proline + oxidized phenazine methosulfate | - |
Pseudomonas fluorescens | (S)-1-pyrroline-5-carboxylate + reduced phenazine methosulfate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRODH | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on | Pseudomonas fluorescens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas fluorescens | isoelectric focussing | - |
6.97 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | ProDH is a negatively charged protein | Pseudomonas fluorescens |
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Release 2023.1 (January 2023)
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