Literature summary for 1.5.99.B2 extracted from

Tallarita, E.; Pollegioni, L.; Servi, S.; Molla, G.
Expression in Escherichia coli of the catalytic domain of human proline oxidase (2012), Protein Expr. Purif., 82, 345-351.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Lactate	the activity is fully inhibited at 5 mM lactate	Homo sapiens
additional information	no inhibition is observed up to 50 mM L-pipecolate	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.47	-	L-proline	in 10 mM MOPS, pH 7.5, at 25°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial membrane	inner mitochondrial membrane	Homo sapiens	31966	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	2 * 46000, SDS-PAGE	Homo sapiens
46276	-	2 * 46276, calculated from amino acid sequence	Homo sapiens
94900	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O	-	Homo sapiens	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 46000, SDS-PAGE	Homo sapiens
homodimer	2 * 46276, calculated from amino acid sequence	Homo sapiens

Synonyms

Synonyms	Comment	Organism
proline oxidase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Homo sapiens

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Release 2023.1 (January 2023)