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Literature summary for 1.5.99.B2 extracted from

  • Omidinia, E.; Mahdizadehdehosta, R.; Mohammadi, H.
    Expression, purification and characterization of the proline dehydrogenase domain of PutA from Pseudomonas putida POS-F84 (2013), Indian J. Microbiol., 53, 297-302.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.99.B2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21 (DE3) plysS cells Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
31
-
 L-proline in 100 mM Tris–HCl, 10 mM MgCl2, 10% (v/v) glycerol, pH 8.5, at 25°C Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida POS-F84
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ NTA affinity column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O weak activity Pseudomonas putida ?
-
 ?
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O weak activity Pseudomonas putida POS-F84 ?
-
 ?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
-
 Pseudomonas putida DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
 ?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
-
 Pseudomonas putida POS-F84 DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
 ?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O weak activity Pseudomonas putida ?
-
 ?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O weak activity Pseudomonas putida POS-F84 ?
-
 ?
additional information D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates Pseudomonas putida ?
-
 ?
additional information D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates Pseudomonas putida POS-F84 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
L-proline:FAD oxidoreductase
-
 Pseudomonas putida
PRODH
-
 Pseudomonas putida
PutA shows proline dehydrogenase activity Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 the enzyme loses a considerable amount of its original activity at above 30°C and is nearly inactivated at 70°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD required Pseudomonas putida
additional information NAD+ and NADP+ are inert Pseudomonas putida