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Literature summary for 1.5.99.B2 extracted from

  • Mohammadi, H.; Omidinia, E.
    Proline dehydrogenase from Pseudomonas fluorescens: Gene cloning, purification, characterization and homology modeling (2012), Appl. Biochem. Microbiol., 48, 167-174.
    View publication on PubMed
Search for more literature for 1.5.99.B2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21 plysS (DE3) cells Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
35
-
 L-proline at pH 8.5 and 30°C Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens Pf-5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + 2,6-dichlorophenolindophenol + H2O 52% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
glycine + 2,6-dichlorophenolindophenol + H2O 52% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-alanine + 2,6-dichlorophenolindophenol + H2O 48% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-alanine + 2,6-dichlorophenolindophenol + H2O 48% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-arginine + 2,6-dichlorophenolindophenol + H2O 62% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-arginine + 2,6-dichlorophenolindophenol + H2O 62% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-histidine + 2,6-dichlorophenolindophenol + H2O 72% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-histidine + 2,6-dichlorophenolindophenol + H2O 72% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O 100% activity compared to L-proline Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
L-leucine + 2,6-dichlorophenolindophenol + H2O 42% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-proline + 2,6-dichlorophenolindophenol + H2O 100% activity Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
 Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
 ?
L-serine + 2,6-dichlorophenolindophenol + H2O 55% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-threonine + 2,6-dichlorophenolindophenol + H2O 66% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
L-valine + 2,6-dichlorophenolindophenol + H2O 33% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate Pseudomonas fluorescens ?
-
 ?
additional information no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate Pseudomonas fluorescens Pf-5 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
L-proline: FAD oxidoreductase
-
 Pseudomonas fluorescens
PRODH
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas fluorescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 more than 60% activity between 20-40°C, a sharp decrease is observed above 30°C and enzyme activity is completely inactivated at 70°C Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas fluorescens

pH Range

pH Minimum pH Maximum Comment Organism
6 11 more than 50% activity between pH 6.0 and 11.0 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas fluorescens