Literature summary for 1.5.99.B2 extracted from

White, T.A.; Johnson, W.H.; Whitman, C.P.; Tanner, J.J.
Structural basis for the inactivation of Thermus thermophilus proline dehydrogenase by N-propargylglycine (2008), Biochemistry, 47, 5573-5580.

Search for more literature for 1.5.99.B2

Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, with 100 mM imidazole buffer at pH 7, 100 mM MgCl2, 14% 2-methyl-2,4-pentanediol, and 5 mM dithiothreitol	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
N-propargylglycine	-	Thermus thermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Thermus thermophilus	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37980	-	native enzyme, calculated from sequence of cDNA	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q72IB8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O	-	Thermus thermophilus	(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?

Synonyms

Synonyms	Comment	Organism
PRODH	-	Thermus thermophilus
proline dehydrogenase	-	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Thermus thermophilus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.2	-	N-propargylglycine	at 25°C	Thermus thermophilus

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Release 2023.1 (January 2023)