Literature summary for 1.5.99.B2 extracted from

Kawakami, R.; Sakuraba, H.; Tsuge, H.; Goda, S.; Katunuma, N.; Ohshima, T.
A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 (2005), FEBS J., 272, 4044-4054.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.03	-	2,6-dichlorophenolindophenol	isoform PDH1	Pyrococcus horikoshii
4	-	L-proline	isoform PDH1	Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
8000	-	isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE	Pyrococcus horikoshii
20000	-	isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE	Pyrococcus horikoshii
43000	-	isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE	Pyrococcus horikoshii
46000	-	isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE	Pyrococcus horikoshii
52000	-	isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE	Pyrococcus horikoshii
56000	-	isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE	Pyrococcus horikoshii
101000	-	isoform PDH2, gel filtration	Pyrococcus horikoshii
440000	-	isoform PDH1, gel filtration	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	isoforms PDH1 and PDH2	-
Pyrococcus horikoshii OT-3	-	isoforms PDH1 and PDH2	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.939	-	isoform PDH1	Pyrococcus horikoshii
1.4	-	isoform PDH2	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O	-	Pyrococcus horikoshii	(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	-	Pyrococcus horikoshii OT-3	(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
additional information	isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid	Pyrococcus horikoshii	?	-	?
additional information	isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid	Pyrococcus horikoshii OT-3	?	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE	Pyrococcus horikoshii
octamer	isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE	Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	isoform PDH1, stable for at least 120 min	Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	isoform PDH1	Pyrococcus horikoshii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	10	isoform PDH1, 50°C, stable for at least 30 min	Pyrococcus horikoshii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	isoform PDH1, 1 mol per mol of alpha subunit	Pyrococcus horikoshii
FAD	isoform PDH1, 1 mol per mol of beta subunit, alpha and beta subunits separatively produced in Escherichia coli cannot bind FAD	Pyrococcus horikoshii
FMN	situated at the interface of alpha and beta subunit	Pyrococcus horikoshii

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Release 2023.1 (January 2023)