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Literature summary for 1.5.99.6 extracted from
- Identification of flavin adenine dinucleotide and heme in a homogeneous spermidine dehydrogenase from Serratia marcescens (1970), J. Biol. Chem., 245, 5424-5433.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
sedimentation equilibrium centrifugation, centrifugation in a sucrose density gradient | Serratia marcescens |
| 79000 | - |
sedimentation velocity centrifugation, diffusion coefficient | Serratia marcescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| spermidine + cytochrome c | Serratia marcescens | natural electron acceptor horse heart cytochrome c | 1,3-diaminopropane + gamma-aminobutylaldehyde | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | - |
ATCC 25179 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 420 | - |
- |
Serratia marcescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, can be stored in 0.1 M potassium phosphate buffer, pH 7.2 for over 6 months with less than 10% loss of activity | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| monoacetylspermidine + electron acceptor | - |
Serratia marcescens | ? + reduced electron acceptor | - |
? | |
| N,N'-bis(3-aminopropyl)-1,3-propanediamine + electron acceptor | - |
Serratia marcescens | ? + reduced electron acceptor | - |
? | |
| N-(3-aminopropyl)-1,3-propanediamine + electron acceptor | - |
Serratia marcescens | ? + reduced electron acceptor | - |
? | |
| N-(3-hydroxypropyl)-1,4-diaminobutane + electron acceptor | - |
Serratia marcescens | ? + reduced electron acceptor | - |
? | |
| spermidine + 2,6-dichlorophenolindophenol | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol | - |
? | |
| spermidine + cytochrome c | natural electron acceptor horse heart cytochrome c | Serratia marcescens | 1,3-diaminopropane + gamma-aminobutylaldehyde | - |
? | |
| spermidine + electron acceptor | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor | - |
? | |
| spermidine + K3Fe(CN)6 | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide | - |
? | |
| spermidine + phenazine methosulfate | - |
Serratia marcescens | 1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate | - |
? | |
| spermine + electron acceptor | - |
Serratia marcescens | ? + reduced electron acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 76000, SDS-PAGE | Serratia marcescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
stable when heated for 5 min | Serratia marcescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Serratia marcescens | |
| heme | - |
Serratia marcescens | |
| iron-protoporphyrin IX | - |
Serratia marcescens | |
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Release 2023.1 (January 2023)
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