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Literature summary for 1.5.99.5 extracted from

  • Chen, Y.; Scanlan, J.; Song, L.; Crombie, A.; Rahman, M.T.; Schaefer, H.; Murrell, J.C.
    gamma-Glutamylmethylamide is an essential intermediate in the metabolism of methylamine by Methylocella silvestris (2010), Appl. Environ. Microbiol., 76, 4530-4537.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.99.5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-methyl-L-glutamate + acceptor + H2O Methylocella silvestris
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Methylocella silvestris BL2
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Methylocella silvestris
-
-
-
Methylocella silvestris BL2
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:monomethylamine-grown cell
-
 Methylocella silvestris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methyl-L-glutamate + acceptor + H2O
-
 Methylocella silvestris L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Methylocella silvestris BL2 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O the enzyme shows monomethylamine dehydrogenase activity with methylamine hydrochloride as substrate, assay using 2,6-dichlorophenolindophenol (DCPIP) as an artificial electron acceptor with or without KCN Methylocella silvestris L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O the enzyme shows monomethylamine dehydrogenase activity with methylamine hydrochloride as substrate, assay using 2,6-dichlorophenolindophenol (DCPIP) as an artificial electron acceptor with or without KCN Methylocella silvestris BL2 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

Synonyms Comment Organism
NMG dehydrogenase
-
 Methylocella silvestris
NMGDH
-
 Methylocella silvestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Methylocella silvestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Methylocella silvestris

Expression

Organism Comment Expression
Methylocella silvestris the enzyme is induced by growth of the cells on monomethylamine, induction of an eight-gene cluster (Msil2632 to Msil2639) by monomethylamine and cotranscription as an operon up

General Information

General Information Comment Organism
metabolism the bacterium utilizes a methylated amino acid pathway gamma-glutamylmethylamide and N-methylglutamate for monomethylamine metabolism. The two key enzymes in this pathway are gamma-glutamylmethylamide synthetase and N-methylglutamate dehydrogenase. The gamma-glutamylmethylamide synthetase is essential for the pathway Methylocella silvestris