Solubilization and partial purification of N-methylglutamate dehydrogenase from Pseudomonas aminovorans
Bamforth, C.W.; Large, P.J.; Biochem. Soc. Trans. 3, 1066-1069 (1975)
No PubMed abstract available
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
deoxycholate
-
Aminobacter aminovorans
Lubrol Px
-
Aminobacter aminovorans
Triton X-100
-
Aminobacter aminovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
phenazine methosulfate
-
Aminobacter aminovorans
0.019
-
2,6-dichlorophenolindophenol
-
Aminobacter aminovorans
0.025
-
N-Methyl-L-glutamate
-
Aminobacter aminovorans
0.11
-
horse heart cytochrome c
-
Aminobacter aminovorans
0.46
-
N-methyl DL-aspartate
-
Aminobacter aminovorans
87
-
N-methyl DL-alanine
-
Aminobacter aminovorans
90.9
-
sarcosine
-
Aminobacter aminovorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
membrane
-
Aminobacter aminovorans
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Pseudomonas sp.
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
N-methyl-L-glutamate + acceptor + H2O
Aminobacter aminovorans
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
N-methyl-L-glutamate + acceptor + H2O
Pseudomonas sp. MA / M.A. / ATCC 23819
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans AM1
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Aminobacter aminovorans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.002
-
-
Aminobacter aminovorans
Storage Stability
Storage Stability
Organism
-15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml
Aminobacter aminovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
-
437744
Pseudomonas sp.
?
-
-
-
?
additional information
L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+
437744
Aminobacter aminovorans
?
-
-
-
?
additional information
L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+
437744
Aminobacter aminovorans AM1
?
-
-
-
?
additional information
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
?
-
-
-
?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans AM1
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437744
Pseudomonas sp.
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methylglutamate + horse heart cytochrome c + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced horse heart cytochrome c
-
-
-
ir
N-methylglutamate + horse heart cytochrome c + H2O
-
437744
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced horse heart cytochrome c
-
-
-
ir
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp.
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Pseudomonas sp.
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp.
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Aminobacter aminovorans
7
8.5
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
deoxycholate
-
Aminobacter aminovorans
Lubrol Px
-
Aminobacter aminovorans
Triton X-100
-
Aminobacter aminovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
phenazine methosulfate
-
Aminobacter aminovorans
0.019
-
2,6-dichlorophenolindophenol
-
Aminobacter aminovorans
0.025
-
N-Methyl-L-glutamate
-
Aminobacter aminovorans
0.11
-
horse heart cytochrome c
-
Aminobacter aminovorans
0.46
-
N-methyl DL-aspartate
-
Aminobacter aminovorans
87
-
N-methyl DL-alanine
-
Aminobacter aminovorans
90.9
-
sarcosine
-
Aminobacter aminovorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
membrane
-
Aminobacter aminovorans
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Pseudomonas sp.
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
N-methyl-L-glutamate + acceptor + H2O
Aminobacter aminovorans
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
N-methyl-L-glutamate + acceptor + H2O
Pseudomonas sp. MA / M.A. / ATCC 23819
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Aminobacter aminovorans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.002
-
-
Aminobacter aminovorans
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml
Aminobacter aminovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
-
437744
Pseudomonas sp.
?
-
-
-
?
additional information
L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+
437744
Aminobacter aminovorans
?
-
-
-
?
additional information
L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+
437744
Aminobacter aminovorans AM1
?
-
-
-
?
additional information
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
?
-
-
-
?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans AM1
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437744
Pseudomonas sp.
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methylglutamate + horse heart cytochrome c + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced horse heart cytochrome c
-
-
-
ir
N-methylglutamate + horse heart cytochrome c + H2O
-
437744
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced horse heart cytochrome c
-
-
-
ir
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp.
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Pseudomonas sp.
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp.
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437744
Aminobacter aminovorans
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437744
Pseudomonas sp. MA / M.A. / ATCC 23819
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Aminobacter aminovorans
7
8.5
-
Pseudomonas sp.
Other publictions for EC 1.5.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700286
Latypova
Genetics of the glutamate-medi ...
Methyloversatilis universalis, Methyloversatilis universalis FAM5
Mol. Microbiol.
75
426-439
2010
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723999
Chen
gamma-Glutamylmethylamide is a ...
Methylocella silvestris, Methylocella silvestris BL2
Appl. Environ. Microbiol.
76
4530-4537
2010
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
1
-
-
4
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
437750
Biville
-
Physiological properties of a ...
Methylobacterium organophilum
FEMS Microbiol. Lett.
52
53-58
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
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-
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-
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1
-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437749
Janvier
-
Methylophaga marina gen nov., ...
Methylophaga marina, Methylophaga thalassica
Int. J. Syst. Bacteriol.
35
131-139
1985
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
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-
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-
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-
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-
-
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2
-
-
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437748
Boulton
-
N-Methylglutamate dehydrogenas ...
bacterium AT2, Hyphomicrobium vulgare, Pseudomonas sp., Aminobacter aminovorans, Pseudomonas methylica, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819, Hyphomicrobium vulgare ZV, Hyphomicrobium vulgare 3
J. Gen. Microbiol.
117
293-304
1980
-
-
-
-
-
-
-
2
4
-
2
7
-
18
-
-
2
-
-
-
6
-
39
2
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
4
-
2
7
-
-
-
2
-
-
6
-
39
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
437747
Bamforth
The kinetic mechanism of N-met ...
Aminobacter aminovorans
Biochem. Soc. Trans.
6
193-195
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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-
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-
-
-
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1
-
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1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437745
Bamforth
Solubilization, partial purifi ...
Aminobacter aminovorans, Aminobacter aminovorans AM1, Aminobacter aminovorans N.C.I.B. 9039
Biochem. J.
161
357-370
1977
-
-
-
-
-
-
6
7
1
1
-
1
-
13
-
-
1
-
-
-
5
2
19
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
6
-
7
1
1
-
1
-
-
-
1
-
-
5
2
19
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
437746
Bamforth
The molecular size of N-methyl ...
Aminobacter aminovorans
Biochem. J.
167
509-512
1977
-
-
-
-
-
-
-
-
1
-
2
1
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
437744
Bamforth
-
Solubilization and partial pur ...
Pseudomonas sp., Aminobacter aminovorans, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819
Biochem. Soc. Trans.
3
1066-1069
1975
-
-
-
-
-
-
3
7
2
-
-
3
-
13
-
-
1
-
-
-
1
1
23
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
7
2
-
-
3
-
-
-
1
-
-
1
1
23
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
437743
Hersh
N-methylglutamate dehydrogenas ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
150
219-226
1972
-
-
-
-
-
-
4
12
1
-
-
1
-
9
-
-
1
-
-
-
-
1
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
12
1
-
-
1
-
-
-
1
-
-
-
1
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437742
Hersh
An N-methyl glutamate dehydrog ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
145
115-120
1971
-
-
-
-
-
-
-
1
1
-
-
2
-
9
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
2
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-