BRENDA - Enzyme Database
show all sequences of 1.5.99.5

N-methylglutamate dehydrogenase: kinetic studies on the solubilized enzyme

Hersh, L.B.; Stark, M.J.; Worthen, S.; Fiero, M.K.; Arch. Biochem. Biophys. 150, 219-226 (1972) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-ketoglutarate
-
Pseudomonas sp.
formaldehyde
product inhibition
Pseudomonas sp.
glutamate
product inhibition
Pseudomonas sp.
p-chloromercuriphenylsulfonate
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
phenazine methosulfate
-
Pseudomonas sp.
0.043
-
N-Methyl-L-glutamate
-
Pseudomonas sp.
0.054
-
2,6-dichlorophenolindophenol
-
Pseudomonas sp.
0.1
-
2-(methylamino)acetic acid
-
Pseudomonas sp.
0.2
-
N-methyl DL-valine
-
Pseudomonas sp.
0.2
-
N-methyl L-isoleucine
-
Pseudomonas sp.
1.3
-
N-methyl L-aspartate
-
Pseudomonas sp.
1.4
-
potassium ferricyanide
-
Pseudomonas sp.
2.5
-
N-methyl L-phenylalanine
-
Pseudomonas sp.
6
-
N-methyl L-alanine
-
Pseudomonas sp.
36
-
N-methyl L-serine
-
Pseudomonas sp.
200
-
sarcosine
-
Pseudomonas sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Pseudomonas sp.
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-15°C, several months
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
N,N-dimethylglutamate is no substrate
437743
Pseudomonas sp.
?
-
-
-
?
additional information
N,N-dimethylglutamate is no substrate
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
?
-
-
-
?
N-methyl DL-valine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
DL-valine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-isoleucine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-isoleucine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-phenylalanine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-phenylalanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-serine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-serine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-glycine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methylglutamate + cytochrome c + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced cytochrome c
-
-
-
?
N-methylglutamate + cytochrome c + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced cytochrome c
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + potassium ferricyanide + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced potassium ferricyanide
-
-
-
?
N-methylglutamate + potassium ferricyanide + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced potassium ferricyanide
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-ketoglutarate
-
Pseudomonas sp.
formaldehyde
product inhibition
Pseudomonas sp.
glutamate
product inhibition
Pseudomonas sp.
p-chloromercuriphenylsulfonate
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
phenazine methosulfate
-
Pseudomonas sp.
0.043
-
N-Methyl-L-glutamate
-
Pseudomonas sp.
0.054
-
2,6-dichlorophenolindophenol
-
Pseudomonas sp.
0.1
-
2-(methylamino)acetic acid
-
Pseudomonas sp.
0.2
-
N-methyl DL-valine
-
Pseudomonas sp.
0.2
-
N-methyl L-isoleucine
-
Pseudomonas sp.
1.3
-
N-methyl L-aspartate
-
Pseudomonas sp.
1.4
-
potassium ferricyanide
-
Pseudomonas sp.
2.5
-
N-methyl L-phenylalanine
-
Pseudomonas sp.
6
-
N-methyl L-alanine
-
Pseudomonas sp.
36
-
N-methyl L-serine
-
Pseudomonas sp.
200
-
sarcosine
-
Pseudomonas sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Pseudomonas sp.
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, several months
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
N,N-dimethylglutamate is no substrate
437743
Pseudomonas sp.
?
-
-
-
?
additional information
N,N-dimethylglutamate is no substrate
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
?
-
-
-
?
N-methyl DL-valine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
DL-valine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-isoleucine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-isoleucine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-phenylalanine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-phenylalanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-serine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-serine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-glycine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methylglutamate + cytochrome c + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced cytochrome c
-
-
-
?
N-methylglutamate + cytochrome c + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced cytochrome c
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + potassium ferricyanide + H2O
-
437743
Pseudomonas sp.
L-glutamate + formaldehyde + reduced potassium ferricyanide
-
-
-
?
N-methylglutamate + potassium ferricyanide + H2O
-
437743
Pseudomonas sp. MA / M.A. / ATCC 23819
L-glutamate + formaldehyde + reduced potassium ferricyanide
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437743
Pseudomonas sp.
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Other publictions for EC 1.5.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
700286
Latypova
Genetics of the glutamate-medi ...
Methyloversatilis universalis, Methyloversatilis universalis FAM5
Mol. Microbiol.
75
426-439
2010
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4
1
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2
2
-
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-
723999
Chen
gamma-Glutamylmethylamide is a ...
Methylocella silvestris, Methylocella silvestris BL2
Appl. Environ. Microbiol.
76
4530-4537
2010
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2
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7
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1
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4
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2
1
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1
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2
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1
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4
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
437750
Biville
-
Physiological properties of a ...
Methylobacterium organophilum
FEMS Microbiol. Lett.
52
53-58
1988
-
-
-
-
-
-
-
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1
-
1
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1
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1
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-
437749
Janvier
-
Methylophaga marina gen nov., ...
Methylophaga marina, Methylophaga thalassica
Int. J. Syst. Bacteriol.
35
131-139
1985
-
-
-
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2
-
2
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2
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2
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437748
Boulton
-
N-Methylglutamate dehydrogenas ...
bacterium AT2, Hyphomicrobium vulgare, Pseudomonas sp., Aminobacter aminovorans, Pseudomonas methylica, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819, Hyphomicrobium vulgare ZV, Hyphomicrobium vulgare 3
J. Gen. Microbiol.
117
293-304
1980
-
-
-
-
-
-
-
2
4
-
2
7
-
18
-
-
2
-
-
-
6
-
39
2
-
-
-
1
-
1
-
-
3
-
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-
-
-
3
-
-
-
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-
-
2
4
-
2
7
-
-
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2
-
-
6
-
39
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
437747
Bamforth
The kinetic mechanism of N-met ...
Aminobacter aminovorans
Biochem. Soc. Trans.
6
193-195
1978
-
-
-
-
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1
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1
-
3
-
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2
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-
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437745
Bamforth
Solubilization, partial purifi ...
Aminobacter aminovorans, Aminobacter aminovorans AM1, Aminobacter aminovorans N.C.I.B. 9039
Biochem. J.
161
357-370
1977
-
-
-
-
-
-
6
7
1
1
-
1
-
13
-
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1
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-
-
5
2
19
-
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1
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1
1
1
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1
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6
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7
1
1
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1
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1
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5
2
19
-
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1
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1
1
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-
-
-
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437746
Bamforth
The molecular size of N-methyl ...
Aminobacter aminovorans
Biochem. J.
167
509-512
1977
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1
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2
1
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3
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1
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437744
Bamforth
-
Solubilization and partial pur ...
Pseudomonas sp., Aminobacter aminovorans, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819
Biochem. Soc. Trans.
3
1066-1069
1975
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3
7
2
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3
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13
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1
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1
1
23
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2
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1
23
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2
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437743
Hersh
N-methylglutamate dehydrogenas ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
150
219-226
1972
-
-
-
-
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4
12
1
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1
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9
-
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1
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1
19
-
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4
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12
1
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1
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1
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1
19
-
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437742
Hersh
An N-methyl glutamate dehydrog ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
145
115-120
1971
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1
1
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9
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