Literature summary for 1.5.99.4 extracted from

Li, H.; Xie, K.; Yu, W.; Hu, L.; Huang, H.; Xie, H.; Wang, S.
Nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in Agrobacterium tumefaciens S33 (2016), Appl. Environ. Microbiol., 82, 1745-1755 .

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains a [2Fe-2S]-center	Agrobacterium tumefaciens

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	-	-	-
Agrobacterium tumefaciens S33	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration	Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O	-	Agrobacterium tumefaciens	(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol	-	?
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O	-	Agrobacterium tumefaciens S33	(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 75000 + 1 * 15000, SDS-PAGE	Agrobacterium tumefaciens
heterodimer	1 * 82400 + 1 * 17100, calculated from amino acid sequence	Agrobacterium tumefaciens

Synonyms

Synonyms	Comment	Organism
NdhAB	-	Agrobacterium tumefaciens

Cofactor

Cofactor	Comment	Organism	Structure
molybdopterin	-	Agrobacterium tumefaciens
[2Fe-2S]-center	-	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)