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Literature summary for 1.5.99.4 extracted from

  • Igloi, G.L.; Brandsch, R.
    Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system (2003), J. Bacteriol., 185, 1976-1986.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.99.4

Cloned(Commentary)

Cloned (Comment) Organism
gene 6hdno of pAO1, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km of (S)-nicotine uptake is 0.0056 mM Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-nicotine + acceptor + H2O Paenarthrobacter nicotinovorans
-
 (R)-6-hydroxynicotine + reduced acceptor
-
 ?
additional information Paenarthrobacter nicotinovorans organism can grow on L-nicotine since it is converted to D-nicotine by a racemase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans Q59127 30 kDa; strain bearing the catabolic plasmid pAO1, gene 6hdno encodes the D-nicotine inducible and specific isozyme
-
Paenarthrobacter nicotinovorans Q59128 small 17.8 kDa subunit; strain bearing the catabolic plasmid pAO1, gene 6hdno encodes the D-nicotine inducible and specific isozyme
-
Paenarthrobacter nicotinovorans Q59129 87.7 kDa; strain bearing the catabolic plasmid pAO1, gene 6hdno encodes the D-nicotine inducible and specific isozyme
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:citrate-grown cell
-
 Paenarthrobacter nicotinovorans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-nicotine + acceptor + H2O
-
 Paenarthrobacter nicotinovorans (R)-6-hydroxynicotine + reduced acceptor
-
 ?
additional information organism can grow on L-nicotine since it is converted to D-nicotine by a racemase Paenarthrobacter nicotinovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
6-hydroxy-D-nicotine oxidase
-
 Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
molybdenum dinucleotide cofactor
-
 Paenarthrobacter nicotinovorans