Literature summary for 1.5.99.4 extracted from

Jones, M.V.
Cytochrome c linked nicotinic acid hydroxylase in Pseudomonas ovalis chester (1973), FEBS Lett., 32, 321-324.

Search for more literature for 1.5.99.4

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	stimulates slightly	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
2,2'-dipyridyl	76% inhibition at 5 mM	Pseudomonas putida
8-hydroxyquinoline	77% inhibition at 5 mM	Pseudomonas putida
Cu2+	88% inhibition at 0.1 mM	Pseudomonas putida
hydroxylamine	22% inhibition at 5 mM	Pseudomonas putida
p-hydroxymercuribenzoate	92% inhibition at 1 mM	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	Chester	-
Pseudomonas putida Chester	-	Chester	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1096	-	reduction of 2,6-dichlorophenolindophenol	Pseudomonas putida

Storage Stability

Storage Stability	Organism
room temperature, EDTA prevents gradual loss of activity	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nicotinic acid + acceptor + H2O	2,6-dichlorophenolindophenol used as electron acceptor	Pseudomonas putida	6-hydroxynicotinic acid + reduced acceptor	-	?
nicotinic acid + acceptor + H2O	2,6-dichlorophenolindophenol used as electron acceptor	Pseudomonas putida Chester	6-hydroxynicotinic acid + reduced acceptor	-	?

Synonyms

Synonyms	Comment	Organism
nicotinic acid hydroxylase	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome c	-	Pseudomonas putida

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Release 2023.1 (January 2023)