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Literature summary for 1.5.99.4 extracted from
- The purification and properties of nicotine oxidase (1967), Biochim. Biophys. Acta, 139, 56-68.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 47% inhibition at 2 mM | Pseudarthrobacter oxydans |  |
| 2,2'-dipyridyl | 27% inhibition at 2 mM | Pseudarthrobacter oxydans | |
| Acriflavine | 64% inhibition at 1 mM | Pseudarthrobacter oxydans | |
| brilliant cresyl blue | concentration over 0.2 mM | Pseudarthrobacter oxydans | |
| Cu2+ | - |
Pseudarthrobacter oxydans | |
| cyanide | 95% inhibition at 1 mM potassium cyanide | Pseudarthrobacter oxydans | |
| Hg2+ | 96% inhibition at 0.1 mM | Pseudarthrobacter oxydans | |
| iodoacetamide | 23% inhibition at 10 mM | Pseudarthrobacter oxydans | |
| additional information | riboflavin 5'-phosphate protects enzyme against acriflavine inhibition, FMN protects enzyme against acriflavine inhibition, FAD only partially effective, glutathione protects against p-chloromercuriphenylsulfonic inhibition | Pseudarthrobacter oxydans | |
| p-chloromercuriphenylsulfonic acid | 62% inhibition at 1 mM | Pseudarthrobacter oxydans | |
| Quinacrine | 49% inhibition at 0.1 mM | Pseudarthrobacter oxydans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
brilliant cresyl blue | - |
Pseudarthrobacter oxydans | |
| 0.027 | - |
methylene blue | - |
Pseudarthrobacter oxydans | |
| 0.037 | - |
Nicotine | - |
Pseudarthrobacter oxydans | |
| 0.49 | - |
menadione | - |
Pseudarthrobacter oxydans | |
| 1 | - |
vitamin K5 | - |
Pseudarthrobacter oxydans | |
| 1.24 | - |
2,6-dichlorophenolindophenol | - |
Pseudarthrobacter oxydans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibitory | Pseudarthrobacter oxydans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudarthrobacter oxydans | - |
P-34 DSM 419 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, heat denaturation, TEAE-cellulose chromatography | Pseudarthrobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.2 | - |
- |
Pseudarthrobacter oxydans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -19°C, 1 month, without thawing and refreezing, without loss of activity | Pseudarthrobacter oxydans |
| -19°C, cells, 4 months, without loss of activity | Pseudarthrobacter oxydans |
| 4°C, 2-3 days, no significant loss of activity | Pseudarthrobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-nicotine + 2,6-dichlorophenolindophenol + H2O | - |
Pseudarthrobacter oxydans | (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol | - |
r | |
| (S)-nicotine + brilliant cresyl blue + H2O | - |
Pseudarthrobacter oxydans | (S)-6-hydroxynicotine + reduced brilliant cresyl blue | - |
r | |
| (S)-nicotine + menadione + H2O | - |
Pseudarthrobacter oxydans | (S)-6-hydroxynicotine + menadiol | - |
r | |
| (S)-nicotine + methylene blue + H2O | - |
Pseudarthrobacter oxydans | (S)-6-hydroxynicotine + reduced methylene blue | - |
r | |
| (S)-nicotine + vitamin K5 + H2O | - |
Pseudarthrobacter oxydans | (S)-6-hydroxynicotine + reduced vitamin K5 | - |
r | |
| anabasine + acceptor + H2O | - |
Pseudarthrobacter oxydans | 6-hydroxyanabasine + reduced acceptor | - |
? | |
| myosmine + acceptor + H2O | - |
Pseudarthrobacter oxydans | ? + reduced acceptor | - |
? | |
| nicotine + acceptor + H2O | brilliant cresyl blue, methylene blue, 5-hydroxy-1,4-naphthoquinone, 2,6-dichlorophenolindophenol, menadione and vitamin K5 used as electron acceptors | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
| nicotine-N-oxide + acceptor + H2O | - |
Pseudarthrobacter oxydans | 6-hydroxynicotine-N-oxide + reduced acceptor | - |
? | |
| nicotinic acid + acceptor + H2O | - |
Pseudarthrobacter oxydans | 6-hydroxynicotinic acid + reduced acceptor | - |
? | |
| nornicotine + acceptor + H2O | - |
Pseudarthrobacter oxydans | 6-hydroxynornicotine + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudarthrobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.2 | sodium diphosphate buffer | Pseudarthrobacter oxydans |
| 7.9 | - |
assay at | Pseudarthrobacter oxydans |
| 8.6 | 9 | Tris chloride or Tris phosphate buffer | Pseudarthrobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | partially protects the enzyme | Pseudarthrobacter oxydans | |
| FMN | metalloflavoprotein, protects enzyme against inhibition by acriflavine | Pseudarthrobacter oxydans | |
| additional information | metalloflavoprotein | Pseudarthrobacter oxydans |
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