BRENDA - Enzyme Database show
show all sequences of 1.5.99.4

The purification and properties of nicotine oxidase

Hochstein, L.L.; Dalton, B.P.; Biochim. Biophys. Acta 139, 56-68 (1967)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
47% inhibition at 2 mM
Pseudarthrobacter oxydans
2,2'-dipyridyl
27% inhibition at 2 mM
Pseudarthrobacter oxydans
Acriflavine
64% inhibition at 1 mM
Pseudarthrobacter oxydans
brilliant cresyl blue
concentration over 0.2 mM
Pseudarthrobacter oxydans
Cu2+
-
Pseudarthrobacter oxydans
cyanide
95% inhibition at 1 mM potassium cyanide
Pseudarthrobacter oxydans
Hg2+
96% inhibition at 0.1 mM
Pseudarthrobacter oxydans
iodoacetamide
23% inhibition at 10 mM
Pseudarthrobacter oxydans
additional information
riboflavin 5'-phosphate protects enzyme against acriflavine inhibition, FMN protects enzyme against acriflavine inhibition, FAD only partially effective, glutathione protects against p-chloromercuriphenylsulfonic inhibition
Pseudarthrobacter oxydans
p-Chloromercuriphenylsulfonic acid
62% inhibition at 1 mM
Pseudarthrobacter oxydans
Quinacrine
49% inhibition at 0.1 mM
Pseudarthrobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
brilliant cresyl blue
-
Pseudarthrobacter oxydans
0.027
-
methylene blue
-
Pseudarthrobacter oxydans
0.037
-
Nicotine
-
Pseudarthrobacter oxydans
0.49
-
menadione
-
Pseudarthrobacter oxydans
1
-
vitamin K5
-
Pseudarthrobacter oxydans
1.24
-
2,6-dichlorophenolindophenol
-
Pseudarthrobacter oxydans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
inhibitory
Pseudarthrobacter oxydans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudarthrobacter oxydans
-
P-34 DSM 419
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, heat denaturation, TEAE-cellulose chromatography
Pseudarthrobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
29.2
-
-
Pseudarthrobacter oxydans
Storage Stability
Storage Stability
Organism
-19°C, 1 month, without thawing and refreezing, without loss of activity
Pseudarthrobacter oxydans
-19°C, cells, 4 months, without loss of activity
Pseudarthrobacter oxydans
4°C, 2-3 days, no significant loss of activity
Pseudarthrobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
anabasine + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxyanabasine + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
myosmine + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
? + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nicotine + acceptor + H2O
brilliant cresyl blue, methylene blue, 5-hydroxy-1,4-naphthoquinone, 2,6-dichlorophenolindophenol, menadione and vitamin K5 used as electron acceptors
392538
Pseudarthrobacter oxydans
6-hydroxynicotine + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nicotine-N-oxide + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxynicotine-N-oxide + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nicotinic acid + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxynicotinic acid + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nornicotine + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxynornicotine + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudarthrobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
8.2
sodium diphosphate buffer
Pseudarthrobacter oxydans
7.9
-
assay at
Pseudarthrobacter oxydans
8.6
9
Tris chloride or Tris phosphate buffer
Pseudarthrobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
partially protects the enzyme
Pseudarthrobacter oxydans
FMN
metalloflavoprotein, protects enzyme against inhibition by acriflavine
Pseudarthrobacter oxydans
additional information
metalloflavoprotein
Pseudarthrobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
partially protects the enzyme
Pseudarthrobacter oxydans
FMN
metalloflavoprotein, protects enzyme against inhibition by acriflavine
Pseudarthrobacter oxydans
additional information
metalloflavoprotein
Pseudarthrobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
47% inhibition at 2 mM
Pseudarthrobacter oxydans
2,2'-dipyridyl
27% inhibition at 2 mM
Pseudarthrobacter oxydans
Acriflavine
64% inhibition at 1 mM
Pseudarthrobacter oxydans
brilliant cresyl blue
concentration over 0.2 mM
Pseudarthrobacter oxydans
Cu2+
-
Pseudarthrobacter oxydans
cyanide
95% inhibition at 1 mM potassium cyanide
Pseudarthrobacter oxydans
Hg2+
96% inhibition at 0.1 mM
Pseudarthrobacter oxydans
iodoacetamide
23% inhibition at 10 mM
Pseudarthrobacter oxydans
additional information
riboflavin 5'-phosphate protects enzyme against acriflavine inhibition, FMN protects enzyme against acriflavine inhibition, FAD only partially effective, glutathione protects against p-chloromercuriphenylsulfonic inhibition
Pseudarthrobacter oxydans
p-Chloromercuriphenylsulfonic acid
62% inhibition at 1 mM
Pseudarthrobacter oxydans
Quinacrine
49% inhibition at 0.1 mM
Pseudarthrobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
brilliant cresyl blue
-
Pseudarthrobacter oxydans
0.027
-
methylene blue
-
Pseudarthrobacter oxydans
0.037
-
Nicotine
-
Pseudarthrobacter oxydans
0.49
-
menadione
-
Pseudarthrobacter oxydans
1
-
vitamin K5
-
Pseudarthrobacter oxydans
1.24
-
2,6-dichlorophenolindophenol
-
Pseudarthrobacter oxydans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
inhibitory
Pseudarthrobacter oxydans
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, heat denaturation, TEAE-cellulose chromatography
Pseudarthrobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
29.2
-
-
Pseudarthrobacter oxydans
Storage Stability (protein specific)
Storage Stability
Organism
-19°C, 1 month, without thawing and refreezing, without loss of activity
Pseudarthrobacter oxydans
-19°C, cells, 4 months, without loss of activity
Pseudarthrobacter oxydans
4°C, 2-3 days, no significant loss of activity
Pseudarthrobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
anabasine + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxyanabasine + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
myosmine + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
? + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nicotine + acceptor + H2O
brilliant cresyl blue, methylene blue, 5-hydroxy-1,4-naphthoquinone, 2,6-dichlorophenolindophenol, menadione and vitamin K5 used as electron acceptors
392538
Pseudarthrobacter oxydans
6-hydroxynicotine + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nicotine-N-oxide + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxynicotine-N-oxide + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nicotinic acid + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxynicotinic acid + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
nornicotine + acceptor + H2O
-
392538
Pseudarthrobacter oxydans
6-hydroxynornicotine + reduced acceptor
-
392538
Pseudarthrobacter oxydans
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudarthrobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
8.2
sodium diphosphate buffer
Pseudarthrobacter oxydans
7.9
-
assay at
Pseudarthrobacter oxydans
8.6
9
Tris chloride or Tris phosphate buffer
Pseudarthrobacter oxydans
Other publictions for EC 1.5.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724032
Qiu
Cloning of a novel nicotine ox ...
no activity in Escherichia coli strain DH5alpha, no activity in Pseudomonas putida strain KT2440, Pseudomonas sp., Pseudomonas sp. HZN6
Appl. Environ. Microbiol.
79
2164-2171
2013
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684760
Ganas
Two closely related pathways o ...
Nocardioides sp., Paenarthrobacter nicotinovorans
Arch. Microbiol.
189
511-517
2008
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671467
Sachelaru
A functional mobA gene for mol ...
Paenarthrobacter nicotinovorans
Appl. Environ. Microbiol.
72
5126-5131
2006
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655850
Igloi
Sequence of the 165-kilobase c ...
Paenarthrobacter nicotinovorans
J. Bacteriol.
185
1976-1986
2003
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656187
Sandu
Characterization of HdnoR, the ...
Paenarthrobacter nicotinovorans
J. Biol. Chem.
278
51307-51315
2003
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392430
Schenk
Gene structures and properties ...
Paenarthrobacter nicotinovorans
J. Mol. Biol.
284
1323-1339
1998
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392431
Grether-Beck
Structural analysis and molybd ...
Paenarthrobacter nicotinovorans
Mol. Microbiol.
13
929-936
1994
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392540
Williams
Rabbit nasal cytochrome P-450 ...
Oryctolagus cuniculus
Biochem. Biophys. Res. Commun.
166
945-952
1990
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391423
Nagel
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Bactopterin as component of eu ...
Pseudarthrobacter oxydans
FEMS Microbiol. Lett.
60
323-326
1989
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392537
Freudenberg
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Nicotine dehydrogenase from Ar ...
Pseudarthrobacter oxydans
FEMS Microbiol. Lett.
52
13-18
1988
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392541
Nakayama
Heterogeneity of hepatic nicot ...
Rattus norvegicus
Biochim. Biophys. Acta
715
254-257
1982
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392542
Jones
Cytochrome c linked nicotinic ...
Pseudomonas putida, Pseudomonas putida Chester
FEBS Lett.
32
321-324
1973
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392538
Hochstein
The purification and propertie ...
Pseudarthrobacter oxydans
Biochim. Biophys. Acta
139
56-68
1967
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392440
Decker
Induction and purification of ...
Pseudarthrobacter oxydans
Biochim. Biophys. Acta
105
313-324
1965
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392543
Behrman
The bacterial oxidation of nic ...
Pseudomonas fluorescens, Pseudomonas fluorescens N-9
J. Biol. Chem.
228
923-945
1957
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