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Literature summary for 1.5.99.4 extracted from

  • Freudenberg, W.; Koenig, K.; Andreesen, J.R.
    Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase (1988), FEMS Microbiol. Lett., 52, 13-18.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ four iron groups per mol Pseudarthrobacter oxydans
Molybdenum one molecule per mol Pseudarthrobacter oxydans
additional information two labile sulfur groups per mol Pseudarthrobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15100
-
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE Pseudarthrobacter oxydans
30000
-
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE Pseudarthrobacter oxydans
82000
-
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE Pseudarthrobacter oxydans
120000
-
gel filtration Pseudarthrobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotine + acceptor + H2O Pseudarthrobacter oxydans first enzyme in the nicotine degradation pathway 6-hydroxynicotine + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter oxydans
-
P-34 DSM 419
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ion-exchange, gel filtration Pseudarthrobacter oxydans
hydroxylapatite column Pseudarthrobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.3
-
-
Pseudarthrobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotine + acceptor + H2O preferentially 2,6-dichlorophenolindophenol used as electron acceptor Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
?
nicotine + acceptor + H2O molybdate is required Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
?
nicotine + acceptor + H2O first enzyme in the nicotine degradation pathway Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
?

Subunits

Subunits Comment Organism
trimer 1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE Pseudarthrobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudarthrobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudarthrobacter oxydans

Cofactor

Cofactor Comment Organism Structure
FAD one mol per mol Pseudarthrobacter oxydans
FAD not covalently bound Pseudarthrobacter oxydans
additional information molybdo-iron-sulfur-flavoprotein Pseudarthrobacter oxydans
pterin part of molybdenum cofactor Pseudarthrobacter oxydans