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Literature summary for 1.5.99.4 extracted from

  • Decker, K.; Bleeg, H.
    Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans (1965), Biochim. Biophys. Acta, 105, 313-324.
    View publication on PubMed
Search for more literature for 1.5.99.4

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by any intermediates Pseudarthrobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotine + acceptor + H2O Pseudarthrobacter oxydans first enzyme in the nicotine degradation pathway 6-hydroxynicotine + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate and ammonium sulfate precipitation, gel filtration, ion-exchange Pseudarthrobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.119 units per mg protein, unit is defined as the amount causing a decrease in absorbancy at 578 nm of 0.001 per min Pseudarthrobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotine + acceptor + H2O non-stereospecific enzyme if grown on L-nicotine, assayed with 2,6-dichlorophenolindophenol Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
 ?
nicotine + acceptor + H2O first enzyme in the nicotine degradation pathway Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudarthrobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Pseudarthrobacter oxydans