Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00087 | - |
(S)-nicotine | cosubstrate 2,6-dichlorophenolindophenol, pH 7, 30°C | Agrobacterium tumefaciens | |
0.00124 | - |
2,6-dichlorophenolindophenol | pH 7, 30°C | Agrobacterium tumefaciens | |
0.00167 | - |
(S)-nicotine | cosubstrate pseudoazurin, pH 7, 30°C | Agrobacterium tumefaciens | |
0.00361 | - |
pseudoazurin | pH 7, 30°C | Agrobacterium tumefaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Agrobacterium tumefaciens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens S33 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purifiaction from the periplasmic fraction | Agrobacterium tumefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22 | - |
acceptor 2,6-dichlorophenolindophenol, pH 7, 30°C | Agrobacterium tumefaciens |
110 | - |
acceptor pseudoazurin, pH 7, 30°C | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O | - |
Agrobacterium tumefaciens | (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol | - |
? | |
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O | - |
Agrobacterium tumefaciens S33 | (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol | - |
? | |
(S)-nicotine + pseudoazurin + H2O | acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron | Agrobacterium tumefaciens | (S)-6-hydroxynicotine + reduced pseudoazurin | - |
? | |
(S)-nicotine + pseudoazurin + H2O | acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron | Agrobacterium tumefaciens S33 | (S)-6-hydroxynicotine + reduced pseudoazurin | - |
? | |
additional information | NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors | Agrobacterium tumefaciens | ? | - |
- |
|
additional information | NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors | Agrobacterium tumefaciens S33 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NdhAB | - |
Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
acceptor pseudoazurin | Agrobacterium tumefaciens |
General Information | Comment | Organism |
---|---|---|
physiological function | disruption of the gene encoding pseudoazurin disables nicotine degradation | Agrobacterium tumefaciens |