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Literature summary for 1.5.98.3 extracted from

  • Haase, P.; Deppenmeier, U.; Blaut, M.; Gottschalk, G.
    Purification and characterization of F420H2-dehydrogenase from Methanolobus tindarius (1992), Eur. J. Biochem., 203, 527-531 .
    View publication on PubMed
Search for more literature for 1.5.98.3

General Stability

General Stability Organism
the purified enzyme becomes rapidly inactivated by repeated freezing and thawing Methanolobus tindarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 reduced coenzyme F420 at pH 6.8 and 37°C Methanolobus tindarius

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methanolobus tindarius 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 16 mol non-heme iron per mol enzyme Methanolobus tindarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Methanolobus tindarius

Organism

Organism UniProt Comment Textmining
Methanolobus tindarius
-
-
-
Methanolobus tindarius DSM 2278
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography Methanolobus tindarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced coenzyme F420 + oxidized metronidazole
-
 Methanolobus tindarius oxidized coenzyme F420 + reduced metronidazole
-
 ?
reduced coenzyme F420 + oxidized metronidazole
-
 Methanolobus tindarius DSM 2278 oxidized coenzyme F420 + reduced metronidazole
-
 ?

Subunits

Subunits Comment Organism
? x * 45000 + x * 40000 + x * 22000 + x * 18000 + x * 17000, SDS-PAGE Methanolobus tindarius

Synonyms

Synonyms Comment Organism
F420H2-dehydrogenase
-
 Methanolobus tindarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Methanolobus tindarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.5
-
 reduced coenzyme F420 at pH 6.8 and 37°C Methanolobus tindarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Methanolobus tindarius

pI Value

Organism Comment pI Value Maximum pI Value
Methanolobus tindarius isoelectric focusing
-
 5