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Literature summary for 1.5.98.3 extracted from
- Purification and characterization of F420H2-dehydrogenase from Methanolobus tindarius (1992), Eur. J. Biochem., 203, 527-531 .
General Stability
| General Stability | Organism |
|---|---|
| the purified enzyme becomes rapidly inactivated by repeated freezing and thawing | Methanolobus tindarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0054 | - |
reduced coenzyme F420 | at pH 6.8 and 37°C | Methanolobus tindarius |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methanolobus tindarius | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 16 mol non-heme iron per mol enzyme | Methanolobus tindarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Methanolobus tindarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanolobus tindarius | - |
- |
- |
| Methanolobus tindarius DSM 2278 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography | Methanolobus tindarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced coenzyme F420 + oxidized metronidazole | - |
Methanolobus tindarius | oxidized coenzyme F420 + reduced metronidazole | - |
? | |
| reduced coenzyme F420 + oxidized metronidazole | - |
Methanolobus tindarius DSM 2278 | oxidized coenzyme F420 + reduced metronidazole | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000 + x * 40000 + x * 22000 + x * 18000 + x * 17000, SDS-PAGE | Methanolobus tindarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F420H2-dehydrogenase | - |
Methanolobus tindarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Methanolobus tindarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.5 | - |
reduced coenzyme F420 | at pH 6.8 and 37°C | Methanolobus tindarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Methanolobus tindarius |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanolobus tindarius | isoelectric focusing | - |
5 |
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