Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | stimulates | Methanopyrus kandleri |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5,10-methylenetetrahydromethanopterin | enzyme from Methanopyrus kandleri, pH and temperature not specified in the publication | Methanopyrus kandleri | |
0.03 | - |
5,10-methylenetetrahydromethanopterin | recombinant enzyme expressed in Escherichia coli, pH and temperature not specified in the publication | Methanopyrus kandleri | |
0.04 | - |
oxidized coenzyme F420 | recombinant enzyme expressed in Escherichia coli, pH and temperature not specified in the publication | Methanopyrus kandleri | |
0.08 | - |
oxidized coenzyme F420 | enzyme from Methanopyrus kandleri, pH and temperature not specified in the publication | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31383 | - |
x * 31383, calculated from sequence | Methanopyrus kandleri |
34000 | - |
x * 34000, enzyme from Methanopyrus kandleri | Methanopyrus kandleri |
36000 | - |
x * 36000, recombinant enzyme expressed in Escherichia coli | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | P94951 | - |
- |
Methanopyrus kandleri DSM 6324 | P94951 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | - |
Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | - |
Methanopyrus kandleri DSM 6324 | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31383, calculated from sequence | Methanopyrus kandleri |
? | x * 34000, enzyme from Methanopyrus kandleri | Methanopyrus kandleri |
? | x * 36000, recombinant enzyme expressed in Escherichia coli | Methanopyrus kandleri |
Synonyms | Comment | Organism |
---|---|---|
coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase | - |
Methanopyrus kandleri |
MTD | - |
Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Methanopyrus kandleri |
70 | 80 | - |
Methanopyrus kandleri |