Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanopyrus kandleri |
Crystallization (Comment) | Organism |
---|---|
crystallization trials are performed with the hanging drop vapor diffusion method using a sparse matrix crystallization kit under anaerobic and red light conditions, determination the structures of the Mtd-5,10-methylenetetrahydromethanopterin-, Mtd-5,10-methenyltetrahydromethanopterin- and the Mtd-5,10-methenyltetrahydromethanopterin-F420H2 complexes at 2.1, 2.0, and 1.8 A resolution, both substrate and cofactor bind in a face to face arrangement to an active site cleft, thereby ensuring a direct hydride transfer between their C14a and C5 atoms | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
coenzyme F420 | 1 M (NH4)2SO4, at 65°C | Methanopyrus kandleri | |
0.04 | - |
5,10-methylenetetrahydromethanopterin | 1 M (NH4)2SO4, at 65°C | Methanopyrus kandleri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | Methanopyrus kandleri | hydrogenotrophic methanogenesis pathway in class I methanogens, enzyme of the methanogenic energy metabolism that catalyzes the hydride transfer between substrate and product using coenzyme F420 as hydride carrier | 5,10-methylenetetrahydromethanopterin + coenzyme F420 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | P94951 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Methanopyrus kandleri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3700 | - |
1 M (NH4)2SO4, at 65°C | Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | hydrogenotrophic methanogenesis pathway in class I methanogens, enzyme of the methanogenic energy metabolism that catalyzes the hydride transfer between substrate and product using coenzyme F420 as hydride carrier | Methanopyrus kandleri | 5,10-methylenetetrahydromethanopterin + coenzyme F420 | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | an X-ray structure of methylenetetrahydromethanopterin dehydrogenase at 1.54 A resolution reveals a homohexameric complex organized as a dimer of trimers | Methanopyrus kandleri |
Synonyms | Comment | Organism |
---|---|---|
F420-dependent methylenetetrahydromethanopterin dehydrogenase | - |
Methanopyrus kandleri |
MTD | - |
Methanopyrus kandleri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | - |
Methanopyrus kandleri |