KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
5,10-methylenetetrahydromethanopterin | - |
Methanosarcina barkeri | |
0.018 | - |
coenzyme F420 | - |
Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
6 * 35000, SDS-PAGE | Methanosarcina barkeri |
200000 | - |
gel filtration | Methanosarcina barkeri |
216000 | - |
native PAGE | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | Methanosarcina barkeri | the enzyme is proposed to function in methanol oxidation step to CO2 | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | ternary complex mechanism | Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
670 | - |
- |
Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | - |
Methanosarcina barkeri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | the enzyme is proposed to function in methanol oxidation step to CO2 | Methanosarcina barkeri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 35000, SDS-PAGE | Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.7 | - |
Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | required | Methanosarcina barkeri |