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Literature summary for 1.5.98.1 extracted from

  • te Brommelstroet, B.W.; Geerts, W.J.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.
    Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri (1991), Biochim. Biophys. Acta, 1079, 293-302.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
5,10-methylenetetrahydromethanopterin
-
Methanosarcina barkeri
0.018
-
coenzyme F420
-
Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
6 * 35000, SDS-PAGE Methanosarcina barkeri
200000
-
gel filtration Methanosarcina barkeri
216000
-
native PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 Methanosarcina barkeri the enzyme is proposed to function in methanol oxidation step to CO2 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina barkeri

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 ternary complex mechanism Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
670
-
-
Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
Methanosarcina barkeri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 the enzyme is proposed to function in methanol oxidation step to CO2 Methanosarcina barkeri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 35000, SDS-PAGE Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.7
-
Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
coenzyme F420 required Methanosarcina barkeri