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Literature summary for 1.5.8.4 extracted from

  • Brizio, C.; Brandsch, R.; Douka, M.; Wait, R.; Barile, M.
    The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction (2008), Int. J. Biol. Macromol., 42, 455-462.
    View publication on PubMed
Search for more literature for 1.5.8.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
 His-tagged mMe2GlyDH, SDS-PAGE Rattus norvegicus
94000
-
 His-tagged pMe2GlyDH, SDS-PAGE Rattus norvegicus
97410
-
 pMe2GlyDH, calculated from amino acid sequence Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q63342
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylglycine + acceptor + H2O
-
 Rattus norvegicus sarcosine + formaldehyde + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
mMe2GlyDH mature dimethylglycine dehydrogenase Rattus norvegicus
pMe2GlyDH precursor of dimethylglycine dehydrogenase Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD FAD is covalently bound via an autocatalytic reaction Rattus norvegicus