Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.8.3 extracted from

  • Kato, K.; Walde, P.; Mitsui, H.; Higashi, N.
    Enzymatic activity and stability of D-fructose dehydrogenase and sarcosine dehydrogenase immobilized onto giant vesicles (2003), Biotechnol. Bioeng., 84, 415-423.
    View publication on PubMed
Search for more literature for 1.5.8.3

Activating Compound

Activating Compound Comment Organism Structure
additional information immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles, vesicle preparation overview Pseudomonas putida

General Stability

General Stability Organism
immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 sarcosine pH 7.5, 37°C, immobilized enzyme Pseudomonas putida
1.9
-
 sarcosine pH 7.5, 37°C, non-immobilized enzyme Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas putida 16020
-

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation diagnostic reagent grade Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
storage stability at room temperature is increased for vesicle-immobilized enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + oxidized 1-methoxy-5-methylphenazinium methylsulfate quantification with nitroblue tetrazolium chloride, i.e. NBT or 2,2'-bis(4-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride, giving NBT-formazan in reaction with reduced 1-methoxy-5-methylphenazinium methylsulfate, overview Pseudomonas putida glycine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate
-
 ?

Synonyms

Synonyms Comment Organism
SDH
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
additional information 1-methoxy-5-methylphenazinium methylsulfate acts as electron acceptor Pseudomonas putida