Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles, vesicle preparation overview | Pseudomonas putida |
General Stability | Organism |
---|---|
immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
sarcosine | pH 7.5, 37°C, immobilized enzyme | Pseudomonas putida | |
1.9 | - |
sarcosine | pH 7.5, 37°C, non-immobilized enzyme | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas putida | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | diagnostic reagent grade | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
storage stability at room temperature is increased for vesicle-immobilized enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + oxidized 1-methoxy-5-methylphenazinium methylsulfate | quantification with nitroblue tetrazolium chloride, i.e. NBT or 2,2'-bis(4-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride, giving NBT-formazan in reaction with reduced 1-methoxy-5-methylphenazinium methylsulfate, overview | Pseudomonas putida | glycine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 1-methoxy-5-methylphenazinium methylsulfate acts as electron acceptor | Pseudomonas putida |