Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
C30A | binds FMN non-covalently | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate, wild-type enzyme | Methylophilus methylotrophus | |
0.019 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate, recombinant enzyme | Methylophilus methylotrophus | |
2 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate, mutant C30A | Methylophilus methylotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4Fe-4S-center | Methylophilus methylotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Methylophilus methylotrophus |
to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Methylophilus methylotrophus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + ferricenium hexafluorophosphate | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + ferrocenium hexafluorophosphate | - |
? | |
trimethylamine + H2O + ferricenium hexafluorophosphate | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + ferrocenium hexafluorophosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
trimethylamine | mutant C30A, electron acceptor: ferricenium hexafluorophosphate | Methylophilus methylotrophus | |
38 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate | Methylophilus methylotrophus | |
40 | - |
trimethylamine | recombinant enzyme, electron acceptor: ferricenium hexafluorophosphate | Methylophilus methylotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Methylophilus methylotrophus | |
FMN | covalently bound to Cys30 | Methylophilus methylotrophus |