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Literature summary for 1.5.8.1 extracted from

  • Meiberg, J.B.M.; Harder, W.
    Dimethylamine dehydrogenase from Hyphomicrobium X: purification and some properties of a new enzyme that oxidizes secondary amines (1979), J. Gen. Microbiol., 115, 49-58.
No PubMed abstract available
Search for more literature for 1.5.8.1

General Stability

General Stability Organism
freezing in liquid nitrogen results in a complete loss of activity Hyphomicrobium sp.

Inhibitors

Inhibitors Comment Organism Structure
Dimethylamine 10 mM, 23% inhibition Hyphomicrobium sp.
methylamine above 6 mM, slight inhibition Hyphomicrobium sp.
phenazine methosulfate serves as electron acceptor, maximal activity at 2.4 mM. Above 2.4 mM inhibition Hyphomicrobium sp.
trimethylamine
-
 Hyphomicrobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0156
-
 Dimethylamine
-
 Hyphomicrobium sp.
0.11
-
 N,N,N,N-tetramethyl-o-phenylenediamine
-
 Hyphomicrobium sp.
1.18
-
 phenazine methosulfate
-
 Hyphomicrobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
 2 * 91000, SDS-PAGE Hyphomicrobium sp.
176000
-
 gel filtration Hyphomicrobium sp.

Organism

Organism UniProt Comment Textmining
Hyphomicrobium sp.
-
 X
-
Hyphomicrobium sp. X
-
 X
-

Purification (Commentary)

Purification (Comment) Organism
partial Hyphomicrobium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:dimethylamine-grown cell anaerobically Hyphomicrobium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3638
-
-
 Hyphomicrobium sp.

Storage Stability

Storage Stability Organism
-10°C, 20% v/v ethylene glycol, stable for at least 3 months Hyphomicrobium sp.
0°C, 3 months without stabilizer, 50% loss of activity Hyphomicrobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ethylamine + acetaldehyde + reduced acceptor
-
 ?
diethylamine + H2O + acceptor at 78.3% of the activity with dimethylamine Hyphomicrobium sp. ethylamine + acetaldehyde + reduced acceptor
-
 ?
dimethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. methylamine + formaldehyde + reduced acceptor
-
 ?
dimethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. X methylamine + formaldehyde + reduced acceptor
-
 ?
dimethylamine + H2O + phenazine methosulfate
-
 Hyphomicrobium sp. methylamine + formaldehyde + reduced phenazine methosulfate
-
 r
ethylpropylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ?
-
 ?
ethylpropylamine + H2O + acceptor at 6.8% of the activity with dimethylamine Hyphomicrobium sp. ?
-
 ?
methylethanolamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ?
-
 ?
methylethanolamine + H2O + acceptor at 54.2% of the activity with dimethylamine Hyphomicrobium sp. ?
-
 ?
methylethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ethylamine + formaldehyde + reduced acceptor
-
 ?
methylethylamine + H2O + acceptor at 92.1% of the activity with dimethylamine Hyphomicrobium sp. ethylamine + formaldehyde + reduced acceptor
-
 ?
methylethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. X ethylamine + formaldehyde + reduced acceptor
-
 ?
methylethylamine + H2O + acceptor at 92.1% of the activity with dimethylamine Hyphomicrobium sp. X ethylamine + formaldehyde + reduced acceptor
-
 ?
methylpropylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. propylamine + formaldehyde + reduced acceptor
-
 ?
methylpropylamine + H2O + acceptor at 12.9% of the activity with dimethylamine Hyphomicrobium sp. propylamine + formaldehyde + reduced acceptor
-
 ?
additional information no reaction with cytochrome c Hyphomicrobium sp. ?
-
 ?
additional information no reaction with primary and tertiary amines and quarternary ammonium salts Hyphomicrobium sp. ?
-
 ?
additional information no reaction with cytochrome c Hyphomicrobium sp. X ?
-
 ?
additional information no reaction with primary and tertiary amines and quarternary ammonium salts Hyphomicrobium sp. X ?
-
 ?
N,N,N,N-tetramethyl-o-phenylenediamine + H2O + electron-transfer flavoprotein
-
 Hyphomicrobium sp. ?
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 91000, SDS-PAGE Hyphomicrobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
 Hyphomicrobium sp.