BRENDA - Enzyme Database
show all sequences of 1.5.8.1

Dimethylamine dehydrogenase from Hyphomicrobium X: purification and some properties of a new enzyme that oxidizes secondary amines

Meiberg, J.B.M.; Harder, W.; J. Gen. Microbiol. 115, 49-58 (1979)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
freezing in liquid nitrogen results in a complete loss of activity
Hyphomicrobium sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Dimethylamine
10 mM, 23% inhibition
Hyphomicrobium sp.
methylamine
above 6 mM, slight inhibition
Hyphomicrobium sp.
phenazine methosulfate
serves as electron acceptor, maximal activity at 2.4 mM. Above 2.4 mM inhibition
Hyphomicrobium sp.
trimethylamine
-
Hyphomicrobium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0156
-
Dimethylamine
-
Hyphomicrobium sp.
0.11
-
N,N,N,N-tetramethyl-o-phenylenediamine
-
Hyphomicrobium sp.
1.18
-
phenazine methosulfate
-
Hyphomicrobium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
91000
-
2 * 91000, SDS-PAGE
Hyphomicrobium sp.
176000
-
gel filtration
Hyphomicrobium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hyphomicrobium sp.
-
X
-
Hyphomicrobium sp. X
-
X
-
Purification (Commentary)
Commentary
Organism
partial
Hyphomicrobium sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:dimethylamine-grown cell
anaerobically
Hyphomicrobium sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.3638
-
-
Hyphomicrobium sp.
Storage Stability
Storage Stability
Organism
-10°C, 20% v/v ethylene glycol, stable for at least 3 months
Hyphomicrobium sp.
0°C, 3 months without stabilizer, 50% loss of activity
Hyphomicrobium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
ethylamine + acetaldehyde + reduced acceptor
-
-
-
-
diethylamine + H2O + acceptor
at 78.3% of the activity with dimethylamine
393880
Hyphomicrobium sp.
ethylamine + acetaldehyde + reduced acceptor
-
-
-
-
dimethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
methylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp.
-
dimethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp. X
methylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp. X
-
ethylpropylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
?
-
-
-
-
ethylpropylamine + H2O + acceptor
at 6.8% of the activity with dimethylamine
393880
Hyphomicrobium sp.
?
-
-
-
-
methylethanolamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
?
-
-
-
-
methylethanolamine + H2O + acceptor
at 54.2% of the activity with dimethylamine
393880
Hyphomicrobium sp.
?
-
-
-
-
methylethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp.
-
methylethylamine + H2O + acceptor
at 92.1% of the activity with dimethylamine
393880
Hyphomicrobium sp.
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp.
-
methylethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp. X
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp. X
-
methylethylamine + H2O + acceptor
at 92.1% of the activity with dimethylamine
393880
Hyphomicrobium sp. X
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp. X
-
methylpropylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
propylamine + formaldehyde + reduced acceptor
-
-
-
-
methylpropylamine + H2O + acceptor
at 12.9% of the activity with dimethylamine
393880
Hyphomicrobium sp.
propylamine + formaldehyde + reduced acceptor
-
-
-
-
additional information
no reaction with cytochrome c
393880
Hyphomicrobium sp.
?
-
-
-
-
additional information
no reaction with primary and tertiary amines and quarternary ammonium salts
393880
Hyphomicrobium sp.
?
-
-
-
-
additional information
no reaction with cytochrome c
393880
Hyphomicrobium sp. X
?
-
-
-
-
additional information
no reaction with primary and tertiary amines and quarternary ammonium salts
393880
Hyphomicrobium sp. X
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 91000, SDS-PAGE
Hyphomicrobium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
-
Hyphomicrobium sp.
General Stability (protein specific)
General Stability
Organism
freezing in liquid nitrogen results in a complete loss of activity
Hyphomicrobium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Dimethylamine
10 mM, 23% inhibition
Hyphomicrobium sp.
methylamine
above 6 mM, slight inhibition
Hyphomicrobium sp.
phenazine methosulfate
serves as electron acceptor, maximal activity at 2.4 mM. Above 2.4 mM inhibition
Hyphomicrobium sp.
trimethylamine
-
Hyphomicrobium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0156
-
Dimethylamine
-
Hyphomicrobium sp.
0.11
-
N,N,N,N-tetramethyl-o-phenylenediamine
-
Hyphomicrobium sp.
1.18
-
phenazine methosulfate
-
Hyphomicrobium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
91000
-
2 * 91000, SDS-PAGE
Hyphomicrobium sp.
176000
-
gel filtration
Hyphomicrobium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Hyphomicrobium sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:dimethylamine-grown cell
anaerobically
Hyphomicrobium sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.3638
-
-
Hyphomicrobium sp.
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, 20% v/v ethylene glycol, stable for at least 3 months
Hyphomicrobium sp.
0°C, 3 months without stabilizer, 50% loss of activity
Hyphomicrobium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
ethylamine + acetaldehyde + reduced acceptor
-
-
-
-
diethylamine + H2O + acceptor
at 78.3% of the activity with dimethylamine
393880
Hyphomicrobium sp.
ethylamine + acetaldehyde + reduced acceptor
-
-
-
-
dimethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
methylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp.
-
dimethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp. X
methylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp. X
-
ethylpropylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
?
-
-
-
-
ethylpropylamine + H2O + acceptor
at 6.8% of the activity with dimethylamine
393880
Hyphomicrobium sp.
?
-
-
-
-
methylethanolamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
?
-
-
-
-
methylethanolamine + H2O + acceptor
at 54.2% of the activity with dimethylamine
393880
Hyphomicrobium sp.
?
-
-
-
-
methylethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp.
-
methylethylamine + H2O + acceptor
at 92.1% of the activity with dimethylamine
393880
Hyphomicrobium sp.
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp.
-
methylethylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp. X
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp. X
-
methylethylamine + H2O + acceptor
at 92.1% of the activity with dimethylamine
393880
Hyphomicrobium sp. X
ethylamine + formaldehyde + reduced acceptor
-
393880
Hyphomicrobium sp. X
-
methylpropylamine + H2O + acceptor
acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine
393880
Hyphomicrobium sp.
propylamine + formaldehyde + reduced acceptor
-
-
-
-
methylpropylamine + H2O + acceptor
at 12.9% of the activity with dimethylamine
393880
Hyphomicrobium sp.
propylamine + formaldehyde + reduced acceptor
-
-
-
-
additional information
no reaction with cytochrome c
393880
Hyphomicrobium sp.
?
-
-
-
-
additional information
no reaction with primary and tertiary amines and quarternary ammonium salts
393880
Hyphomicrobium sp.
?
-
-
-
-
additional information
no reaction with cytochrome c
393880
Hyphomicrobium sp. X
?
-
-
-
-
additional information
no reaction with primary and tertiary amines and quarternary ammonium salts
393880
Hyphomicrobium sp. X
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 91000, SDS-PAGE
Hyphomicrobium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
-
Hyphomicrobium sp.
Other publictions for EC 1.5.8.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711031
Liffourrena
Pseudomonas putida A ATCC 1263 ...
Pseudomonas putida, Pseudomonas putida ATCC 12633
Arch. Microbiol.
192
471-476
2010
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
2
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
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-
-
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-
2
-
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-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393894
Kim
A novel denitrifying bacterial ...
Paracoccus sp.
Arch. Microbiol.
176
271-277
2001
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
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2
-
6
-
1
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-
1
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2
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2
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4
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2
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6
-
1
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-
1
-
-
-
-
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-
-
-
-
393877
Yang
The primary structure of Hypho ...
Hyphomicrobium sp., Hyphomicrobium sp. X
Eur. J. Biochem.
232
264-271
1995
-
-
1
-
-
-
-
-
-
-
1
-
-
14
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393879
Kasprzak
Identity of the subunits and t ...
Hyphomicrobium sp., Hyphomicrobium sp. X
Biochem. J.
211
535-541
1983
-
-
-
-
-
-
-
-
-
1
-
-
-
13
-
-
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-
-
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-
1
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-
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-
1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393884
Kasprzak
Localization of the major dehy ...
Hyphomicrobium sp., Hyphomicrobium sp. X
J. Bacteriol.
156
348-353
1983
-
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
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-
-
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1
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-
-
393878
Steenkamp
Mechanistic studies on the deh ...
Hyphomicrobium sp., Hyphomicrobium sp. X
Biochem. J.
207
241-252
1982
-
-
-
-
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-
-
1
-
-
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15
-
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
393883
Beinert
-
Dehydrogenases of methylotroph ...
Hyphomicrobium sp., Hyphomicrobium sp. X
Dev. Biochem.
21
727-735
1982
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1
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11
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1
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393880
Meiberg
-
Dimethylamine dehydrogenase fr ...
Hyphomicrobium sp., Hyphomicrobium sp. X
J. Gen. Microbiol.
115
49-58
1979
-
-
-
-
-
1
4
3
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2
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11
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1
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1
1
2
18
1
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1
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1
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4
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3
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2
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1
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1
1
2
18
1
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1
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393881
Steenkamp
Identification of the prosthet ...
Hyphomicrobium sp., Hyphomicrobium sp. X
Biochem. Biophys. Res. Commun.
88
244-250
1979
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1
1
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12
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1
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1
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1
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1
1
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1
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393882
Large
-
Cytochrome cCO is not a primar ...
Hyphomicrobium sp., Hyphomicrobium sp. X
FEMS Microbiol. Lett.
5
281-286
1979
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2
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11
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2
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4
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