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Literature summary for 1.5.7.3 extracted from

  • Yang, T.; Shao, Y.; Guo, L.; Meng, X.; Yu, H.; Lu, W.
    Role of N, N-dimethylglycine and its catabolism to sarcosine in Chromohalobacter salexigens DSM 3043 (2020), Appl. Environ. Microbiol., 86, e01186-20 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.7.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chromohalobacter salexigens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, 33% inhibition Chromohalobacter salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.61
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens
14.85
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, 12% increase in activity Chromohalobacter salexigens
additional information neither inhibitory nor activating: Na+, K+, Zn2+, Mn2+, Mg2+, Fe2+, Co2+ Chromohalobacter salexigens

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QYW1
-
-
Chromohalobacter salexigens DSM 3043 Q1QYW1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, using Ni2x02-chelating affinity chromatography Chromohalobacter salexigens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylglycine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens sarcosine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?
N,N-dimethylglycine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens DSM 3043 sarcosine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?
sarcosine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens glycine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?
sarcosine + 2 oxidized ferredoxin + H2O
-
 Chromohalobacter salexigens DSM 3043 glycine + formaldehyde + 2 reduced ferredoxin + 2 H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 79000, SDS-PAGE Chromohalobacter salexigens

Synonyms

Synonyms Comment Organism
Csal_0990
-
 Chromohalobacter salexigens
DdhC
-
 Chromohalobacter salexigens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Chromohalobacter salexigens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
 64% of maximum activity Chromohalobacter salexigens
80
-
 24% of maximum activity Chromohalobacter salexigens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60 min, 36% residual activity Chromohalobacter salexigens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 sarcosine pH 7.5, 60°C Chromohalobacter salexigens
0.31
-
 N,N-dimethylglycine pH 7.5, 60°C Chromohalobacter salexigens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Chromohalobacter salexigens

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
 Chromohalobacter salexigens

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound Chromohalobacter salexigens
NAD+ prefers NAD+ to NADP+, Km and kcat values are 0.81 mM and 0.20 per sec, respectively Chromohalobacter salexigens
NADP+ prefers NAD+ to NADP+, Km and kcat values are 3.21 mM and 0.14 per sec, respectively Chromohalobacter salexigens

General Information

General Information Comment Organism
physiological function under high-salinity conditions, the presence of dimethylglycine inhibits growth of the wild type and induces the production and accumulation of trehalose and glucosylglycerate intracellularly. Exogenous addition of dimethylglycine significantly improves the growth rates of DdhC mutants incubated at 37°C synthetic medium with sarcosine as the sole N source Chromohalobacter salexigens