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Literature summary for 1.5.7.1 extracted from
- Is reduced ferredoxin the physiological electron donor for MetVF-type methylenetetrahydrofolate reductases in acetogenesis? a hypothesis (2021), Int. Microbiol., 25, 75-88 .
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Clostridium ljungdahlii | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the dimer consisting of two of MetVF heterodimers has 14.9 mol iron per mol enzyme | Clostridium ljungdahlii |  |
| additional information | the dimer consisting of two of MetVF heterodimers has 16.2 mol acid-labile sulfur per mol enzyme | Clostridium ljungdahlii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 106000 | - |
gel filtration | Clostridium ljungdahlii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium ljungdahlii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 57.6fold from cytoplasm by anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration, to homogeneity | Clostridium ljungdahlii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156.1 | - |
purified native enzyme, pH 7.0, 37°C | Clostridium ljungdahlii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyltetrahydrofolate + 2 oxidized ferredoxin | - |
Clostridium ljungdahlii | 5,10-methylenetetrahydrofolate + 2 reduced ferredoxin + 2 H+ | - |
r | |
| additional information | reduced ferredoxin cannot be the physiological electron donor in vivo, since growth by acetogenesis from H2 + CO2 has a negative ATP yield. Reduced methyl viologen is used as electron donor for following the MVred:methylene-THF oxidoreductase activity during the purification procedure. With ethanol as product and Fdred as electron donor for the MetVF-type MTHFR, the ATP yield becomes slightly positive (0.14 ATP/ethanol). But since only minor amounts of ethanol are produced, this does not result in an overall positive ATP yield | Clostridium ljungdahlii | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | (1 * 23000, MetV + 1 * 33000, MetF)2, SDS-PAGE, a dimer consisting of two of MetVF heterodimers | Clostridium ljungdahlii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fdred:methylene-THF oxidoreductase | - |
Clostridium ljungdahlii |
| methylene-tetrahydrofolate reductase | - |
Clostridium ljungdahlii |
| MetVF-type methylenetetrahydrofolate reductase | - |
Clostridium ljungdahlii |
| MetVF-type MTHFR | - |
Clostridium ljungdahlii |
| MTHFR | - |
Clostridium ljungdahlii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium ljungdahlii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 80 | enzyme activity is relatively stable between 37°C and 50°C but decreases to 56% at 20°C and 23% at 80°C | Clostridium ljungdahlii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Clostridium ljungdahlii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | enzyme MTHFR shows maximal activity at pH 7.0 and is significantly less active with only 2% activity left at pH 5.0 and 6% activity left at pH 9.0 | Clostridium ljungdahlii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | the dimer consisting of two of MetVF heterodimers contains 1.87 mol FMN per mol dimeric heterodimer. Reduced ferredoxin cannot be the physiological electron donor in vivo, since growth by acetogenesis from H2 + CO2 has a negative ATP yield | Clostridium ljungdahlii | |
| additional information | NADH and NADPH are not used as electron donor. MTHFR is able to use artificial electron donors for the reduction of methylene-THF | Clostridium ljungdahlii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | type I- and III-MTHFRs are neither involved directly (as terminal electron acceptor) nor indirectly (by producing the fuel for the respiratory enzymes, reduced ferredoxin, by electron bifurcation) in energy conservation. Type IV-MTHFR may be coupled to energy conservation, either directly or indirectly. Modeling of cofactor dependence of enzyme MTHFR, detailed overview | Clostridium ljungdahlii |
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