Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated enzyme containing residues 86-601 and only four Trp residues. Substantial conformational changes of truncated protein upon addition of proline | Escherichia coli |
W194F | mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 50% | Escherichia coli |
W194F/W211F | mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 95% | Escherichia coli |
W211F | mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 80% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
L-proline | truncated mutant W194F/W211F, pH 7.5, 25°C | Escherichia coli | |
56 | - |
L-proline | truncated mutant W194F, pH 7.5, 25°C | Escherichia coli | |
58 | - |
L-proline | truncated wild-type, pH 7.5, 25°C | Escherichia coli | |
59 | - |
L-proline | truncated mutant W211F, pH 7.5, 25°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
enzyme switches from a cytosolic DNA-binding protein to a membrane-bound enzyme with proline dehydrogenase activity and DELTA1-pyrroline-5-carboxylate dehydrogenase activity | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol | switching of enzyme from a cytosolic DNA-binding protein to a membrane-bound enzyme involves W211 in a flexible domain near the proline dehydrogenase active site and occurs in a time scale 10fold lower than the turnover number | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + FAD + H2O | - |
Escherichia coli | (S)-1-pyrroline-5-carboxylate + FADH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PutA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
L-proline | truncated mutant W194F/W211F, pH 7.5, 25°C | Escherichia coli | |
4 | - |
L-proline | truncated mutant W211F, pH 7.5, 25°C | Escherichia coli | |
10 | - |
L-proline | truncated mutant W194F, pH 7.5, 25°C | Escherichia coli | |
20 | - |
L-proline | truncated wild-type, pH 7.5, 25°C | Escherichia coli |