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Literature summary for 1.5.4.1 extracted from

  • Wiederrecht, G.J.; Brown, G.M.
    Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins (1984), J. Biol. Chem., 259, 14121-14127.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0085
-
dihydroneopterin triphosphate
-
Drosophila melanogaster
1.8
-
glutathione
-
Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
2 * 24000, SDS-PAGE Drosophila melanogaster
48000
-
gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-pyruvoyltetrahydropterin + glutathione Drosophila melanogaster enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase glutathione disulfide + pyrimidodiazepine
-
r

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
wild-type flies
-

Purification (Commentary)

Purification (Comment) Organism
-
Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
head
-
Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
-
Drosophila melanogaster

Storage Stability

Storage Stability Organism
-80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months Drosophila melanogaster
-80°C, enzyme purified through the final step stable for at least 1-2 weeks Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-pyruvoyltetrahydropterin + glutathione
-
Drosophila melanogaster glutathione disulfide + pyrimidodiazepine
-
r
6-pyruvoyltetrahydropterin + glutathione enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase Drosophila melanogaster glutathione disulfide + pyrimidodiazepine
-
r
dihydroneopterin triphosphate + glutathione
-
Drosophila melanogaster ?
-
?
glutathione disulfide + pyrimidodiazepine
-
Drosophila melanogaster 6-pyruvoyltetrahydropterin + glutathione
-
r
pyruvoyltetrahydropterin + glutathione
-
Drosophila melanogaster 2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine PDA ?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Drosophila melanogaster