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Literature summary for 1.5.3.7 extracted from
- Characterization of two fructosyl-amino acid oxidase homologs of Schizosaccharomyces pombe (2004), J. Biosci. Bioeng., 97, 278-280.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Schizosaccharomyces pombe |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | 3.5 | L-proline | pH 8.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe |  |
| 4.07 | - |
L-pipecolate | pH 8.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, about, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
| 98000 | - |
recombinant enzyme, gel filtration | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Schizosaccharomyces pombe | enzyme is involved in L-lysine metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
gene FAP1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain JM109 | Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-pipecolate + O2 | highly specific for | Schizosaccharomyces pombe | 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2 | - |
? | |
| L-proline + O2 | low activity | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | enzyme is involved in L-lysine metabolism | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | no activity with fructosyl-amino acids, L-saccharopine, and sarcosine | Schizosaccharomyces pombe | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, about, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fap1 | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Schizosaccharomyces pombe |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | covalently attached | Schizosaccharomyces pombe | |
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Release 2023.1 (January 2023)
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