Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae as a fusion protein between 6-hydroxy-D-nicotine oxidase and the mitochondrial targeting sequence of Neurospora crassa F0-ATPase subunit 9 (Su9-6-HDNO) and translated into rabbit reticulocyte lysate | Paenarthrobacter nicotinovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
fusion protein between 6-hydroxy-D-nicotine oxidase and the mitochondrial targeting sequence of Neurospora crassa F0-ATPase subunit 9 | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | bound as N6-(2-aminoethyl)-FAD 50% activity of the wild type enzyme | Paenarthrobacter nicotinovorans | |
FAD | FAD is bound via an (8alpha)-isoalloxazine-(N3)histidyl linkage | Paenarthrobacter nicotinovorans | |
FAD | formation of the bond to FAD proceeds autocatalytically | Paenarthrobacter nicotinovorans | |
FAD | bound as N6-(6-carboxyhexyl)-FAD 60% activity of the wild type enzyme | Paenarthrobacter nicotinovorans |