Cloned (Comment) | Organism |
---|---|
expression in Sphingomonas aquatilis, Escherichia coli strains are not suitable for expression | Sphingomonas melonis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas melonis | - |
- |
- |
Sphingomonas melonis TY | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-6-hydroxynicotine + H2O + O2 | - |
Sphingomonas melonis | 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2 | - |
? | |
(S)-6-hydroxynicotine + H2O + O2 | - |
Sphingomonas melonis TY | 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NdpB | - |
Sphingomonas melonis |
Organism | Comment | Expression |
---|---|---|
Sphingomonas melonis | expression is induced by nicotine or other nicotine degradation intermediates | up |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme is part of a nicotine-degrading gene cluster, ndp, containing the nicotine hydroxylase, 6-hydroxy-L-nicotine oxidase, 6-hydroxypseudooxynicotine oxidase, and 6-hydroxy-3-succinyl-pyridine monooxygenase responsible for catalyzing the transformation of nicotine to 2,5-dihydropyridine. The linked ndpHFEG genes shared by the variant of the pyridine and pyrrolidine pathways and pyrrolidine pathways indicate that these two pathways might share the same origin | Sphingomonas melonis |
physiological function | after disruption of ndpB gene, the mutant strain loses the ability to grow on nicotine and accumulates 6-hydroxynicotine | Sphingomonas melonis |