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Literature summary for 1.5.3.5 extracted from

  • Wang, S.; Huang, H.; Xie, K.; Xu, P.
    Identification of nicotine biotransformation intermediates by Agrobacterium tumefaciens strain S33 suggests a novel nicotine degradation pathway (2012), Appl. Microbiol. Biotechnol., 95, 1567-1578.
    View publication on PubMed
Search for more literature for 1.5.3.5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2 Agrobacterium tumefaciens
-
 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
 ?
(S)-6-hydroxynicotine + H2O + O2 Agrobacterium tumefaciens S33
-
 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
 ?
additional information Agrobacterium tumefaciens transformation of 6-hydroxy-L-nicotine to 6-hydroxy-N-methylmyosmine, 6-hydroxypseudooxynicotine formation, overview ?
-
 ?
additional information Agrobacterium tumefaciens S33 transformation of 6-hydroxy-L-nicotine to 6-hydroxy-N-methylmyosmine, 6-hydroxypseudooxynicotine formation, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
 isolated from tobacco plant rhizosphere in Shandong Province, China
-
Agrobacterium tumefaciens S33
-
 isolated from tobacco plant rhizosphere in Shandong Province, China
-
Paenarthrobacter nicotinovorans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.462
-
 strain S33, pH 7.0, 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2
-
 Agrobacterium tumefaciens 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
 ?
(S)-6-hydroxynicotine + H2O + O2
-
 Agrobacterium tumefaciens S33 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
 ?
additional information transformation of 6-hydroxy-L-nicotine to 6-hydroxy-N-methylmyosmine, 6-hydroxypseudooxynicotine formation, overview Agrobacterium tumefaciens ?
-
 ?
additional information measurement of enzyme activity using dichlorindophenol. 6-Hydroxypseudooxynicotine forms from 6-hydroxy-N-methylmyosmine non-enzymatically Agrobacterium tumefaciens ?
-
 ?
additional information synthesis of 6-hydroxy-N-methylmyosmine and 6-hydroxy-pseudooxynicotine from 6-hydroxy-L-nicotine. 6-Hydroxypseudooxynicotine forms from 6-hydroxy-N-methylmyosmine non-enzymatically Paenarthrobacter nicotinovorans ?
-
 ?
additional information transformation of 6-hydroxy-L-nicotine to 6-hydroxy-N-methylmyosmine, 6-hydroxypseudooxynicotine formation, overview Agrobacterium tumefaciens S33 ?
-
 ?
additional information measurement of enzyme activity using dichlorindophenol. 6-Hydroxypseudooxynicotine forms from 6-hydroxy-N-methylmyosmine non-enzymatically Agrobacterium tumefaciens S33 ?
-
 ?

Synonyms

Synonyms Comment Organism
6-hydroxy-L-nicotine oxidase
-
 Agrobacterium tumefaciens
6-hydroxy-L-nicotine oxidase
-
 Paenarthrobacter nicotinovorans
6HLNO
-
 Agrobacterium tumefaciens
6HLNO
-
 Paenarthrobacter nicotinovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Agrobacterium tumefaciens
30
-
 assay at Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 assay at Agrobacterium tumefaciens
7 7.5 assay at Paenarthrobacter nicotinovorans

General Information

General Information Comment Organism
metabolism pathways of nicotine degradation by bacteria Paenarthrobacter nicotinovorans
metabolism the enzyme is involved in nicotine degradation, five hydroxylated-pyridine intermediates during the cell growth on nicotine and during transformation of nicotine within resting cells, overview. Agrobacterium strain S33 employs a novel pathway that is different from the two characterized pathways described in Arthrobacter and Pseudomonas. Agrobacterium strain S33 is able to transform nicotine to 6-hydroxypseudooxynicotine first via the pyridine pathway through 6-hydroxy-L-nicotine and 6-hydroxy-N-methylmyosmine, and then, it turns to the pyrrolidine pathway with the formation of 6-hydroxy-3-succinoylpyridine and 2,5-dihydroxypyridine. The cell extract can transform 6-hydroxypseudooxynicotine into 6-hydroxy-3-succinoylpyridine by coupling with 6-hydroxy-Lnicotine oxidation reaction by 6-hydroxy-L-nicotine oxidase. Pathways of nicotine degradation by bacteria,, overview Agrobacterium tumefaciens