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Literature summary for 1.5.3.5 extracted from

  • Dai, V.D.; Decker, K.; Sund, H.
    Purification and properties of L-6-hydroxynicotine oxidase (1968), Eur. J. Biochem., 4, 95-102.
    View publication on PubMed
Search for more literature for 1.5.3.5

Crystallization (Commentary)

Crystallization (Comment) Organism
forms hexagonal crystals in ammonium sulfate solution Paenarthrobacter nicotinovorans

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 100% inhibition at 0.005 mM Paenarthrobacter nicotinovorans
Na2MoO4 12% inhibition at 0.05 mM Paenarthrobacter nicotinovorans
o-phenanthroline 69% inhibition at 9 mM Paenarthrobacter nicotinovorans
p-chloromercuriphenylsulfonate 60% inhibition at 0.025 mM, inhibition can be reversed by an excess of thiol compounds Paenarthrobacter nicotinovorans
Urea at 4 M, 38% inhibition and at 7.2 M, 93% inhibition Paenarthrobacter nicotinovorans
ZnSO4 57% inhibition at 0.05 mM Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, sedimentation equilibrium Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
additional information Paenarthrobacter nicotinovorans enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.73
-
-
 Paenarthrobacter nicotinovorans

Storage Stability

Storage Stability Organism
0°C, saturated ammonium sulfate solution, 2 weeks, little inactivation Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
additional information enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen Paenarthrobacter nicotinovorans ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 47000, sedimentation equilibrium Paenarthrobacter nicotinovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 75% activity after 15 min Paenarthrobacter nicotinovorans
50
-
 9% activity after 5 min Paenarthrobacter nicotinovorans
60
-
 2% activity after 5 min Paenarthrobacter nicotinovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.3
-
 6-hydroxy-L-nicotine
-
 Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Paenarthrobacter nicotinovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 very unstable under pH 6 Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD 1 FAD per subunit, non-covalently bound Paenarthrobacter nicotinovorans