BRENDA - Enzyme Database show
show all sequences of 1.5.3.5

Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans

Schenk, S.; Hoelz, A.; Krauss, B.; Decker, K.; J. Mol. Biol. 284, 1323-1339 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli JM105
Paenarthrobacter nicotinovorans
Crystallization (Commentary)
Crystallization
Organism
-
Paenarthrobacter nicotinovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46265
-
2 * 46265, amino acid analysis
Paenarthrobacter nicotinovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-6-hydroxynicotine + H2O + O2
Paenarthrobacter nicotinovorans
-
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously
Paenarthrobacter nicotinovorans
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paenarthrobacter nicotinovorans
-
formerly Arthrobacter oxidans
-
Purification (Commentary)
Commentary
Organism
-
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-6-hydroxynicotine + H2O + O2
-
392430
Paenarthrobacter nicotinovorans
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously
392430
Paenarthrobacter nicotinovorans
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 46265, amino acid analysis
Paenarthrobacter nicotinovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.2
-
assay at
Paenarthrobacter nicotinovorans
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
1 FAD per subunit, non-covalently bound
Paenarthrobacter nicotinovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli JM105
Paenarthrobacter nicotinovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
1 FAD per subunit, non-covalently bound
Paenarthrobacter nicotinovorans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Paenarthrobacter nicotinovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46265
-
2 * 46265, amino acid analysis
Paenarthrobacter nicotinovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-6-hydroxynicotine + H2O + O2
Paenarthrobacter nicotinovorans
-
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously
Paenarthrobacter nicotinovorans
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-6-hydroxynicotine + H2O + O2
-
392430
Paenarthrobacter nicotinovorans
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously
392430
Paenarthrobacter nicotinovorans
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 46265, amino acid analysis
Paenarthrobacter nicotinovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.2
-
assay at
Paenarthrobacter nicotinovorans
Other publictions for EC 1.5.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741943
Fitzpatrick
Mechanism of flavoprotein L-6 ...
Paenarthrobacter nicotinovorans
Biochemistry
56
869-875
2017
-
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1
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3
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11
-
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1
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1
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1
1
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3
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1
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7
1
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1
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1
1
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3
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11
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1
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1
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-
3
-
1
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-
7
1
-
-
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1
1
-
12
12
741691
Yu
Molecular mechanism of nicoti ...
Ochrobactrum sp. SJY1
Appl. Environ. Microbiol.
81
272-281
2015
-
-
1
-
-
-
-
1
-
1
-
1
-
1
-
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1
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-
-
-
-
2
-
1
1
-
1
1
1
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1
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1
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
1
-
1
1
1
-
-
-
-
-
-
-
-
741686
Qiu
A novel (S)-6-hydroxynicotine ...
Shinella sp.
Appl. Environ. Microbiol.
80
5552-5560
2014
-
-
1
-
-
-
7
2
-
1
-
1
-
4
-
-
1
-
-
-
-
-
3
1
1
1
3
2
1
-
3
1
-
-
-
-
-
1
1
-
-
-
-
7
-
2
-
1
-
1
-
-
-
1
-
-
-
-
3
1
1
1
3
2
1
-
3
-
-
1
1
-
2
2
718677
Wang
Identification of nicotine bio ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens S33, Paenarthrobacter nicotinovorans
Appl. Microbiol. Biotechnol.
95
1567-1578
2012
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
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-
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1
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7
-
2
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-
2
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-
-
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-
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4
-
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-
1
-
7
-
2
-
-
-
2
-
-
-
-
2
2
-
-
-
726378
Kachalova
Crystallographic snapshots of ...
Paenarthrobacter nicotinovorans
Proc. Natl. Acad. Sci. USA
108
4800-4805
2011
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
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-
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4
-
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1
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1
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1
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2
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4
-
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-
-
-
712752
Kachalova
Crystal structure analysis of ...
Paenarthrobacter nicotinovorans
J. Mol. Biol.
396
785-799
2010
-
-
1
1
-
-
1
1
-
-
-
1
-
2
-
1
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
1
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700058
Ganas
Uptake of L-nicotine and of 6- ...
Paenarthrobacter nicotinovorans, Paenarthrobacter nicotinovorans PAO1
Microbiology
155
1866-1877
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
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-
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-
-
-
-
-
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-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
392441
Schenk
Horizontal gene transfer invol ...
Paenarthrobacter nicotinovorans
J. Mol. Evol.
48
178-186
1999
-
-
1
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
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-
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-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
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-
-
-
-
-
-
-
-
-
-
-
392430
Schenk
Gene structures and properties ...
Paenarthrobacter nicotinovorans
J. Mol. Biol.
284
1323-1339
1998
-
-
1
1
-
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-
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1
1
-
3
-
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1
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1
1
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1
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-
1
-
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1
1
1
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-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392431
Grether-Beck
Structural analysis and molybd ...
Paenarthrobacter nicotinovorans
Mol. Microbiol.
13
929-936
1994
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
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-
-
-
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2
1
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1
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1
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-
1
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2
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2
1
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-
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-
392432
Pust
13C, 15N, and 31P NMR studies ...
Paenarthrobacter nicotinovorans
Biochemistry
28
516-521
1989
-
-
-
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1
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2
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2
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2
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1
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1
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1
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2
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2
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392433
Brandsch
6-Hydroxy-D-nicotine oxidase o ...
Paenarthrobacter nicotinovorans
Eur. J. Biochem.
167
315-320
1987
-
-
1
-
-
-
-
-
-
-
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1
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2
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1
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1
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1
1
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1
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-
1
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-
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-
-
-
-
-
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392434
Swafford
Localization of the enantiozym ...
Paenarthrobacter nicotinovorans
J. Bacteriol.
163
792-795
1985
-
-
-
-
-
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1
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1
2
-
2
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2
1
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-
1
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1
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1
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1
2
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-
2
1
-
-
-
-
-
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-
-
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-
-
-
-
-
392435
Hinkkanen
Purification of the flavoprote ...
Paenarthrobacter nicotinovorans
Hoppe-Seyler's Z. Physiol. Chem.
364
801-806
1983
-
-
-
-
-
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
1
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-
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-
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-
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-
-
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-
-
-
392436
Decker
D- and L-6-hydroxynicotine oxi ...
Paenarthrobacter nicotinovorans
Z. Naturforsch. B
27
1072-1073
1972
-
-
-
-
-
-
2
2
-
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1
1
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2
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1
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1
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1
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1
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2
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2
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1
1
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1
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1
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392437
Palmer
-
Mechanisms of flavoprotein cat ...
Paenarthrobacter nicotinovorans
Biol. Oxidations (Singer, T. P. , ed. )
263-300
1968
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-
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1
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1
1
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1
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2
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1
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1
1
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1
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2
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392438
Dai
Purification and properties of ...
Paenarthrobacter nicotinovorans
Eur. J. Biochem.
4
95-102
1968
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-
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1
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6
-
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1
2
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1
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1
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1
1
2
1
-
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3
1
1
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1
1
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1
1
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6
-
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-
1
2
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1
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1
1
2
1
-
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3
1
1
-
1
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392439
Decker
Mechanism and specifcity of L- ...
Paenarthrobacter nicotinovorans
Eur. J. Biochem.
3
132-138
1967
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-
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3
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1
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1
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1
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1
1
2
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1
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3
2
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1
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1
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1
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392440
Decker
Induction and purification of ...
Paenarthrobacter nicotinovorans
Biochim. Biophys. Acta
105
313-324
1965
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1
1
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1
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1
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1
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2
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1
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1
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2
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1
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