Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.3.5 extracted from

  • Schenk, S.; Hoelz, A.; Krauss, B.; Decker, K.
    Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans (1998), J. Mol. Biol., 284, 1323-1339.
    View publication on PubMed
Search for more literature for 1.5.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM105 Paenarthrobacter nicotinovorans

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46265
-
 2 * 46265, amino acid analysis Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously ?

Subunits

Subunits Comment Organism
homodimer 2 * 46265, amino acid analysis Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
 assay at Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD 1 FAD per subunit, non-covalently bound Paenarthrobacter nicotinovorans