Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | stimulates enzyme in prepared mitochondrial fraction, but inhibits activity in crude homogenate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | moderate inhibition | Rattus norvegicus | |
Co2+ | strong inhibitory effect | Rattus norvegicus | |
FAD | moderate inhibition | Rattus norvegicus | |
FMN | moderate inhibition | Rattus norvegicus | |
KCN | 1.3 mM, about 40% inhibition | Rattus norvegicus | |
Mn2+ | moderate inhibition | Rattus norvegicus | |
additional information | not inhibited by semicarbazide | Rattus norvegicus | |
NADH | moderate inhibition | Rattus norvegicus | |
Ni2+ | strong inhibitory effect | Rattus norvegicus | |
phosphate | inhibits activity in crude homogenate, but stimulates enzyme in prepared mitochondrial fraction | Rattus norvegicus | |
Zn2+ | strong inhibitory effect | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
N6,N6-dimethyl-L-lysine | - |
Rattus norvegicus | |
1.63 | - |
N6-methyl-L-lysine | - |
Rattus norvegicus | |
5.95 | - |
N6-ethyl-L-lysine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | 60% of the activity in rat kidney is present in the mitochondrial fraction | Rattus norvegicus | 5739 | - |
particle-bound | - |
Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Cavia porcellus | - |
- |
- |
Columba sp. | - |
- |
- |
Felis catus | - |
low activity | - |
Gallus gallus | - |
- |
- |
Mesocricetus auratus | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2.78fold partial purification | Rattus norvegicus |
enzyme from kidney | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Cavia porcellus | - |
kidney | - |
Mus musculus | - |
kidney | - |
Canis lupus familiaris | - |
kidney | highest activity | Rattus norvegicus | - |
kidney | higher activity than in liver | Gallus gallus | - |
kidney | higher activity than in liver | Rattus norvegicus | - |
kidney | higher activity than in liver | Oryctolagus cuniculus | - |
kidney | higher activity than in liver | Mesocricetus auratus | - |
kidney | higher activity than in liver | Felis catus | - |
kidney | higher activity than in liver | Columba sp. | - |
liver | - |
Mus musculus | - |
liver | - |
Canis lupus familiaris | - |
liver | low activity | Gallus gallus | - |
liver | low activity | Cavia porcellus | - |
liver | low activity | Columba sp. | - |
liver | lower activity than in kidney | Gallus gallus | - |
liver | lower activity than in kidney | Rattus norvegicus | - |
liver | lower activity than in kidney | Oryctolagus cuniculus | - |
liver | lower activity than in kidney | Mesocricetus auratus | - |
liver | lower activity than in kidney | Felis catus | - |
liver | lower activity than in kidney | Columba sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0044 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(methylamino)-2-oxohexanoic acid + H2O + O2 | 15% of the activity with N6-methyl-L-lysine | Rattus norvegicus | 6-amino-2-oxohexanoic acid + formaldehyde + H2O2 | - |
? | |
6-(methylamino)-2-oxohexanoic acid + H2O + O2 | free alpha-amino group is not required for susceptibility | Rattus norvegicus | 6-amino-2-oxohexanoic acid + formaldehyde + H2O2 | - |
? | |
additional information | not: delta-N-monomethyl-L-ornithine | Rattus norvegicus | ? | - |
? | |
additional information | not: several acyl derivatives, alpha-N-methyl-L-lysine | Rattus norvegicus | ? | - |
? | |
additional information | activity restricted to epsilon-N-alkyllysine derivatives | Rattus norvegicus | ? | - |
? | |
N6,N6-dimethyl-L-lysine + O2 + H2O | 46% of the activity with N6-methyl-L-lysine, it may be oxidized by a different enzyme | Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6,N6-dimethyl-L-lysine + O2 + H2O | enzyme consumes per molecule N6,N6-dimethyl-L-lysine one molecule of oxygen | Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-ethyl-L-lysine + H2O + O2 | about 50% of the activity with N6-methyl-L-lysine | Rattus norvegicus | L-lysine + acetaldehyde + H2O2 | - |
? | |
N6-methyl-D-lysine + H2O + O2 | 11% of the activity with epsilon-N-methyl-L-lysine | Rattus norvegicus | D-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Gallus gallus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Cavia porcellus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Mus musculus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Oryctolagus cuniculus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Canis lupus familiaris | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Mesocricetus auratus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Felis catus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | - |
Columba sp. | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | highest enzyme activity | Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen | Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is resistant to heat, far more stable in presence of substrates | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
partially purified enzyme | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.8 | pH 6: about 10% of activity maximum, pH 7.8: about 15% of activity maximum | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme is most likely a flavoprotein | Rattus norvegicus | |
additional information | no cofactor requirement | Rattus norvegicus |