Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition at 0.01 mM | Corynebacterium sp. U-96 | |
acetaldehyde | about 50% inhibition at 20 mM | Corynebacterium sp. U-96 | |
acetate | about 50% inhibition at 20 mM | Corynebacterium sp. U-96 | |
Ag+ | complete inhibition at 2 mM | Corynebacterium sp. U-96 | |
Cd2+ | 7% residual activity at 2 mM | Corynebacterium sp. U-96 | |
Co2+ | 53% residual activity at 2 mM | Corynebacterium sp. U-96 | |
Cu2+ | complete inhibition at 2 mM | Corynebacterium sp. U-96 | |
Fe2+ | 66% residual activity at 2 mM | Corynebacterium sp. U-96 | |
formaldehyde | about 50% inhibition at 20 mM | Corynebacterium sp. U-96 | |
Hg2+ | complete inhibition at 2 mM | Corynebacterium sp. U-96 | |
iodoacetamide | 4% residual activity at 2 mM | Corynebacterium sp. U-96 | |
KCN | 45% residual activity at 2 mM | Corynebacterium sp. U-96 | |
Mn2+ | 63% residual activity at 2 mM | Corynebacterium sp. U-96 | |
additional information | there is no inhibitory effect with metal ions and chemicals such as Ca2+, Mg2+, Fe3+, alpha,alpha'-dipyridyl, EDTA, 2-mercaptoethanol, glutathione (reduced form), dithiothreitol, NaN3, NaF, and NaAsO2 | Corynebacterium sp. U-96 | |
N-ethylmaleimide | 20% residual activity at 2 mM | Corynebacterium sp. U-96 | |
Ni2+ | 76% residual activity at 2 mM | Corynebacterium sp. U-96 | |
o-phenanthroline | 53% residual activity at 2 mM | Corynebacterium sp. U-96 | |
propionate | about 50% inhibition at 20 mM | Corynebacterium sp. U-96 | |
sodium lauryl sulfate | complete inhibition at 2 mM | Corynebacterium sp. U-96 | |
Zn2+ | 7% residual activity at 2 mM | Corynebacterium sp. U-96 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
O2 | at pH 7.7 and 37°C | Corynebacterium sp. U-96 | |
0.31 | - |
2,6-dichlorophenolindophenol | at pH 7.7 and 37°C | Corynebacterium sp. U-96 | |
1.67 | - |
phenazine methosulfate | at pH 7.7 and 37°C | Corynebacterium sp. U-96 | |
2.5 | - |
ferricyanide | at pH 7.7 and 37°C | Corynebacterium sp. U-96 | |
3.4 | - |
sarcosine | at pH 8.3 and 37°C | Corynebacterium sp. U-96 | |
3.8 | - |
sarcosine | at pH 7.0 and 37°C | Corynebacterium sp. U-96 | |
8.7 | - |
N-Methyl-L-alanine | at pH 8.3 and 37°C | Corynebacterium sp. U-96 | |
11.4 | - |
N-ethyl-glycine | at pH 7.0 and 37°C | Corynebacterium sp. U-96 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
174000 | - |
gel filtration | Corynebacterium sp. U-96 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. U-96 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. U-96 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose colum chromatography, QAE-Sephadex A-50 gel filtration, DEAE Sephadex A-50 gel filtration, Ultrogel AcA34 gel filtration, and creatine-AH-Sepharose 4B colum chromatography | Corynebacterium sp. U-96 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.38 | - |
crude enzyme, at pH 8.3 and 37°C | Corynebacterium sp. U-96 |
8.93 | - |
after 23.5fold purification, at pH 8.3 and 37°C | Corynebacterium sp. U-96 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | oxygen cannot be replaced by methylene blue or cytochrome c. The enzyme shows no activity with N,N-dimethylglycine, glycine, betaine, choline, 1,3-dimethylurea, 1-methylguanidine, 1-methylhydantoin, sarcosyl-glycine, and sarcosyl-sarcosine | Corynebacterium sp. U-96 | ? | - |
- |
|
N-ethyl-glycine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | ? | - |
? | |
N-methyl-L-alanine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | ? | - |
? | |
sarcosine + 5,6,7,8-tetrahydrofolate + 2,6-dichlorophenolindophenol | - |
Corynebacterium sp. U-96 | ? | - |
? | |
sarcosine + 5,6,7,8-tetrahydrofolate + ferricyanide | - |
Corynebacterium sp. U-96 | ? | - |
? | |
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? | |
sarcosine + 5,6,7,8-tetrahydrofolate + phenazine methosulfate | - |
Corynebacterium sp. U-96 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 110000 + 1 * 44000 + 1 * 21000 + 1 * 10000, SDS-PAGE | Corynebacterium sp. U-96 |
Synonyms | Comment | Organism |
---|---|---|
sarcosine: oxygen oxidoreductase (demethylating) | - |
Corynebacterium sp. U-96 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in glycylglycine buffer | Corynebacterium sp. U-96 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the enzyme activity is completely lost on heating at 45°C for 10 min | Corynebacterium sp. U-96 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
N-Methyl-L-alanine | at pH 7.0 and 37°C | Corynebacterium sp. U-96 | |
2.1 | - |
N-Methyl-L-alanine | at pH 8.3 and 37°C | Corynebacterium sp. U-96 | |
2.2 | - |
N-ethyl-glycine | at pH 7.0 and 37°C | Corynebacterium sp. U-96 | |
5.8 | - |
sarcosine | at pH 8.3 and 37°C | Corynebacterium sp. U-96 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
in potassium phosphate buffer | Corynebacterium sp. U-96 |
8 | 8.5 | in glycylglycine buffer | Corynebacterium sp. U-96 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the enzyme remains stable for 24 h at pH 7.0-10.0 and 5°C | Corynebacterium sp. U-96 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains both 1 mol of non-covalently bound FAD and 1 mol of covalently bound FAD per mol of enzyme | Corynebacterium sp. U-96 |