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Literature summary for 1.5.3.24 extracted from

  • Moriguchi, T.; Ida, K.; Hikima, T.; Ueno, G.; Yamamoto, M.; Suzuki, H.
    Channeling and conformational changes in the heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96 (2010), J. Biochem., 148, 491-505 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium sp. U-96

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with methylthioacetate, pyrrole 2-carboxylate and sulfite or sarcosine-reduced enzyme, using 0.1 M HEPES (pH 7.5) and 1.7 M ammonium sulfate Corynebacterium sp. U-96

Protein Variants

Protein Variants Comment Organism
Y269A the mutant shows 10% activity compared to the wild type enzyme Corynebacterium sp. U-96
Y271A the mutant shows 0.0076% activity compared to the wild type enzyme Corynebacterium sp. U-96
Y271F the mutant shows 0.78% activity compared to the wild type enzyme Corynebacterium sp. U-96

Inhibitors

Inhibitors Comment Organism Structure
methylthioacetate competitive inhibition with respect to sarcosine Corynebacterium sp. U-96
pyrrole 2-carboxylate competitive inhibition with respect to sarcosine Corynebacterium sp. U-96

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96
11.3
-
sarcosine mutant enzyme Y269A, at pH 8.0 and 25°C Corynebacterium sp. U-96
72.6
-
sarcosine mutant enzyme Y271F, at pH 8.0 and 25°C Corynebacterium sp. U-96
1730
-
sarcosine mutant enzyme Y271A, at pH 8.0 and 25°C Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. U-96
-
glycine + 5,10-methylenetetrahydrofolate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium sp. U-96
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
Corynebacterium sp. U-96 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
?

Subunits

Subunits Comment Organism
heterotetramer 1 * 110000 + 1 * 44000 + 1 * 21000 + 1 * 10000, SDS-PAGE Corynebacterium sp. U-96

Synonyms

Synonyms Comment Organism
heterotetrameric sarcosine oxidase
-
Corynebacterium sp. U-96
sarcosine:oxygen oxidoreductase (demethylating)
-
Corynebacterium sp. U-96

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.65
-
sarcosine mutant enzyme Y271A, at pH 8.0 and 25°C Corynebacterium sp. U-96
7.15
-
sarcosine mutant enzyme Y271F, at pH 8.0 and 25°C Corynebacterium sp. U-96
14.5
-
sarcosine mutant enzyme Y269A, at pH 8.0 and 25°C Corynebacterium sp. U-96
17.5
-
sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96

Cofactor

Cofactor Comment Organism Structure
FAD
-
Corynebacterium sp. U-96
FMN
-
Corynebacterium sp. U-96

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.33
-
pyrrole 2-carboxylate at pH 8.0 and 25°C Corynebacterium sp. U-96
2.05
-
methylthioacetate at pH 8.0 and 25°C Corynebacterium sp. U-96

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00095
-
sarcosine mutant enzyme Y271A, at pH 8.0 and 25°C Corynebacterium sp. U-96
0.098
-
sarcosine mutant enzyme Y271F, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.3
-
sarcosine mutant enzyme Y269A, at pH 8.0 and 25°C Corynebacterium sp. U-96
12.5
-
sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96