Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium sp. U-96 |
Crystallization (Comment) | Organism |
---|---|
enzyme complexed with methylthioacetate, pyrrole 2-carboxylate and sulfite or sarcosine-reduced enzyme, using 0.1 M HEPES (pH 7.5) and 1.7 M ammonium sulfate | Corynebacterium sp. U-96 |
Protein Variants | Comment | Organism |
---|---|---|
Y269A | the mutant shows 10% activity compared to the wild type enzyme | Corynebacterium sp. U-96 |
Y271A | the mutant shows 0.0076% activity compared to the wild type enzyme | Corynebacterium sp. U-96 |
Y271F | the mutant shows 0.78% activity compared to the wild type enzyme | Corynebacterium sp. U-96 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylthioacetate | competitive inhibition with respect to sarcosine | Corynebacterium sp. U-96 | |
pyrrole 2-carboxylate | competitive inhibition with respect to sarcosine | Corynebacterium sp. U-96 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
sarcosine | wild type enzyme, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
11.3 | - |
sarcosine | mutant enzyme Y269A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
72.6 | - |
sarcosine | mutant enzyme Y271F, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
1730 | - |
sarcosine | mutant enzyme Y271A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. U-96 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. U-96 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 110000 + 1 * 44000 + 1 * 21000 + 1 * 10000, SDS-PAGE | Corynebacterium sp. U-96 |
Synonyms | Comment | Organism |
---|---|---|
heterotetrameric sarcosine oxidase | - |
Corynebacterium sp. U-96 |
sarcosine:oxygen oxidoreductase (demethylating) | - |
Corynebacterium sp. U-96 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.65 | - |
sarcosine | mutant enzyme Y271A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
7.15 | - |
sarcosine | mutant enzyme Y271F, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
14.5 | - |
sarcosine | mutant enzyme Y269A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
17.5 | - |
sarcosine | wild type enzyme, at pH 8.0 and 25°C | Corynebacterium sp. U-96 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Corynebacterium sp. U-96 | |
FMN | - |
Corynebacterium sp. U-96 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
pyrrole 2-carboxylate | at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
2.05 | - |
methylthioacetate | at pH 8.0 and 25°C | Corynebacterium sp. U-96 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00095 | - |
sarcosine | mutant enzyme Y271A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
0.098 | - |
sarcosine | mutant enzyme Y271F, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
1.3 | - |
sarcosine | mutant enzyme Y269A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
12.5 | - |
sarcosine | wild type enzyme, at pH 8.0 and 25°C | Corynebacterium sp. U-96 |