Literature summary for 1.5.3.24 extracted from

Eschenbrenner, M.; Chlumsky, L.J.; Khanna, P.; Strasser, F.; Jorns, M.S.
Organization of the multiple coenzymes and subunits and role of the covalent flavin link in the complex heterotetrameric sarcosine oxidase (2001), Biochemistry, 40, 5352-5367 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Corynebacterium sp. P-1

Protein Variants

Protein Variants	Comment	Organism
C141S	the mutant exhibits specific activity values reduced to those of the wild type recombinant enzyme or the natural enzyme	Corynebacterium sp. P-1
C195S	the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme	Corynebacterium sp. P-1
C351S	the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme	Corynebacterium sp. P-1
G139A	the mutant of the alpha subunit is expressed only at low temperature (15°C), but the purified enzyme exhibits properties indistinguishable from the wild type enzyme	Corynebacterium sp. P-1
G30A	the mutant of the beta subunit exhibits subunit expression levels similar to those of the wild type enzyme, but the mutation blocks subunit assembly and covalent attachment of FMN	Corynebacterium sp. P-1
H173N	catalytically inactive mutant of the beta subunit	Corynebacterium sp. P-1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2	Corynebacterium sp. P-1	-	glycine + 5,10-methylenetetrahydrofolate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium sp. P-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
phenyl-Sepharose column chromatography and Talon affinity resin column chromatography	Corynebacterium sp. P-1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methylthioacetate + 5,6,7,8-tetrahydrofolate + O2	-	Corynebacterium sp. P-1	?	-	?
sarcosine + 5,6,7,8-tetrahydrofolate + O2	-	Corynebacterium sp. P-1	glycine + 5,10-methylenetetrahydrofolate + H2O2	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	-	Corynebacterium sp. P-1

Synonyms

Synonyms	Comment	Organism
heterotetrameric sarcosine oxidase	-	Corynebacterium sp. P-1
TSOX	-	Corynebacterium sp. P-1

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Corynebacterium sp. P-1
FMN	-	Corynebacterium sp. P-1
NAD+	-	Corynebacterium sp. P-1

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Release 2023.1 (January 2023)