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Literature summary for 1.5.3.22 extracted from

  • Seedorf, H.; Dreisbach, A.; Hedderich, R.; Shima, S.; Thauer, R.K.
    F420H2 oxidase (FprA) from Methanobrevibacter arboriphilus, a coenzyme F420-dependent enzyme involved in O2 detoxification (2004), Arch. Microbiol., 182, 126-137.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Methanothermobacter marburgensis
-
Methanobrevibacter arboriphilus

Inhibitors

Inhibitors Comment Organism Structure
additional information the reaction is not inhibited by cyanide Methanobrevibacter arboriphilus
additional information the reaction is not inhibited by cyanide Methanothermobacter marburgensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
O2 pH 7.6, 37°C Methanothermobacter marburgensis
0.002
-
O2 pH 7.6, 37°C Methanobrevibacter arboriphilus
0.03
-
reduced coenzyme F420 pH 7.6, 37°C Methanothermobacter marburgensis
0.03
-
reduced coenzyme F420 pH 7.6, 37°C Methanobrevibacter arboriphilus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the 45000 Da protein harbors a binuclear iron center Methanobrevibacter arboriphilus
Iron the 45000 Da protein harbors a binuclear iron center Methanothermobacter marburgensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Methanothermobacter marburgensis
45000
-
x * 45000, SDS-PAGE Methanobrevibacter arboriphilus
45300
-
x * 45300, calculated from sequence Methanothermobacter marburgensis
46100
-
x * 46100, calculated from sequence Methanobrevibacter arboriphilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 reduced coenzyme F420 + O2 Methanothermobacter marburgensis the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 Methanobrevibacter arboriphilus the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 Methanothermobacter marburgensis DSM 2133 the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 Methanobrevibacter arboriphilus DSM Z 744 the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Methanobrevibacter arboriphilus B1A7S3
-
-
Methanobrevibacter arboriphilus DSM Z 744 B1A7S3
-
-
Methanothermobacter marburgensis Q50497
-
-
Methanothermobacter marburgensis DSM 2133 Q50497
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter marburgensis
-
Methanobrevibacter arboriphilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
pH 7.6, 37°C, cell suspension Methanobrevibacter arboriphilus
55
-
pH 7.6, 37°C, purified enzyme Methanobrevibacter arboriphilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanothermobacter marburgensis 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanobrevibacter arboriphilus 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanothermobacter marburgensis 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanobrevibacter arboriphilus 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanothermobacter marburgensis DSM 2133 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanothermobacter marburgensis DSM 2133 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanobrevibacter arboriphilus DSM Z 744 2 oxidized coenzyme F420 + 2 H2O
-
?
2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanobrevibacter arboriphilus DSM Z 744 2 oxidized coenzyme F420 + 2 H2O
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Methanothermobacter marburgensis
? x * 45000, SDS-PAGE Methanobrevibacter arboriphilus
? x * 45300, calculated from sequence Methanothermobacter marburgensis
? x * 46100, calculated from sequence Methanobrevibacter arboriphilus

Synonyms

Synonyms Comment Organism
FprA
-
Methanothermobacter marburgensis
FprA
-
Methanobrevibacter arboriphilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanobrevibacter arboriphilus
65
-
assay at Methanothermobacter marburgensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Methanothermobacter marburgensis
7.5
-
-
Methanobrevibacter arboriphilus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 30% of the maximal activity at pH 6.5 and 8.5 Methanobrevibacter arboriphilus

Cofactor

Cofactor Comment Organism Structure
FMN the 45000 Da protein contains 1 FMN per mol Methanothermobacter marburgensis
FMN the 45000 Da protein contains 1 FMN per mol Methanobrevibacter arboriphilus

pI Value

Organism Comment pI Value Maximum pI Value
Methanobrevibacter arboriphilus calculated from sequence
-
5.3

General Information

General Information Comment Organism
physiological function the enzyme is involved in O2 detoxification Methanothermobacter marburgensis
physiological function the enzyme is involved in O2 detoxification Methanobrevibacter arboriphilus