Cloned (Comment) | Organism |
---|---|
- |
Methanothermobacter marburgensis |
- |
Methanobrevibacter arboriphilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the reaction is not inhibited by cyanide | Methanobrevibacter arboriphilus | |
additional information | the reaction is not inhibited by cyanide | Methanothermobacter marburgensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
O2 | pH 7.6, 37°C | Methanothermobacter marburgensis | |
0.002 | - |
O2 | pH 7.6, 37°C | Methanobrevibacter arboriphilus | |
0.03 | - |
reduced coenzyme F420 | pH 7.6, 37°C | Methanothermobacter marburgensis | |
0.03 | - |
reduced coenzyme F420 | pH 7.6, 37°C | Methanobrevibacter arboriphilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the 45000 Da protein harbors a binuclear iron center | Methanobrevibacter arboriphilus | |
Iron | the 45000 Da protein harbors a binuclear iron center | Methanothermobacter marburgensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Methanothermobacter marburgensis |
45000 | - |
x * 45000, SDS-PAGE | Methanobrevibacter arboriphilus |
45300 | - |
x * 45300, calculated from sequence | Methanothermobacter marburgensis |
46100 | - |
x * 46100, calculated from sequence | Methanobrevibacter arboriphilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced coenzyme F420 + O2 | Methanothermobacter marburgensis | the enzyme is involved in O2 detoxification | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | Methanobrevibacter arboriphilus | the enzyme is involved in O2 detoxification | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | Methanothermobacter marburgensis DSM 2133 | the enzyme is involved in O2 detoxification | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | Methanobrevibacter arboriphilus DSM Z 744 | the enzyme is involved in O2 detoxification | 2 oxidized coenzyme F420 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobrevibacter arboriphilus | B1A7S3 | - |
- |
Methanobrevibacter arboriphilus DSM Z 744 | B1A7S3 | - |
- |
Methanothermobacter marburgensis | Q50497 | - |
- |
Methanothermobacter marburgensis DSM 2133 | Q50497 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter marburgensis |
- |
Methanobrevibacter arboriphilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
pH 7.6, 37°C, cell suspension | Methanobrevibacter arboriphilus |
55 | - |
pH 7.6, 37°C, purified enzyme | Methanobrevibacter arboriphilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced coenzyme F420 + O2 | the enzyme is involved in O2 detoxification | Methanothermobacter marburgensis | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme is involved in O2 detoxification | Methanobrevibacter arboriphilus | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor | Methanothermobacter marburgensis | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor | Methanobrevibacter arboriphilus | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme is involved in O2 detoxification | Methanothermobacter marburgensis DSM 2133 | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor | Methanothermobacter marburgensis DSM 2133 | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme is involved in O2 detoxification | Methanobrevibacter arboriphilus DSM Z 744 | 2 oxidized coenzyme F420 + 2 H2O | - |
? | |
2 reduced coenzyme F420 + O2 | the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor | Methanobrevibacter arboriphilus DSM Z 744 | 2 oxidized coenzyme F420 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Methanothermobacter marburgensis |
? | x * 45000, SDS-PAGE | Methanobrevibacter arboriphilus |
? | x * 45300, calculated from sequence | Methanothermobacter marburgensis |
? | x * 46100, calculated from sequence | Methanobrevibacter arboriphilus |
Synonyms | Comment | Organism |
---|---|---|
FprA | - |
Methanothermobacter marburgensis |
FprA | - |
Methanobrevibacter arboriphilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanobrevibacter arboriphilus |
65 | - |
assay at | Methanothermobacter marburgensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanothermobacter marburgensis |
7.5 | - |
- |
Methanobrevibacter arboriphilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | 30% of the maximal activity at pH 6.5 and 8.5 | Methanobrevibacter arboriphilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | the 45000 Da protein contains 1 FMN per mol | Methanothermobacter marburgensis | |
FMN | the 45000 Da protein contains 1 FMN per mol | Methanobrevibacter arboriphilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanobrevibacter arboriphilus | calculated from sequence | - |
5.3 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in O2 detoxification | Methanothermobacter marburgensis |
physiological function | the enzyme is involved in O2 detoxification | Methanobrevibacter arboriphilus |