BRENDA - Enzyme Database
show all sequences of 1.5.3.19

A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1

Chiribau, C.; Sandu, C.; Fraaije, M.; Schiltz, E.; Brandsch, R.; Eur. J. Biochem. 271, 4677-4684 (2004) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Paenarthrobacter nicotinovorans
Engineering
Protein Variants
Commentary
Organism
H67A
mutation results in resulted in a protein without covalently bound flavin. Mutant protein contains noncovalently bound flavin and exhibits 10% of the enzyme activity of the wild-type
Paenarthrobacter nicotinovorans
W66S
mutation results in resulted in a protein without covalently bound flavin and complete loss of actiovity
Paenarthrobacter nicotinovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
4-methylaminobutanoate
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
25
-
sarcosine
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
89395
-
1 * 90000, SDS-PAGE, 1 * 89395, calculated
Paenarthrobacter nicotinovorans
90000
-
gel filtration
Paenarthrobacter nicotinovorans
Organism
Organism
UniProt
Commentary
Textmining
Paenarthrobacter nicotinovorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-methylaminobutanoate + O2 + H2O
-
719401
Paenarthrobacter nicotinovorans
4-aminobutanoate + formaldehyde + H2O2
-
-
-
?
additional information
no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid
719401
Paenarthrobacter nicotinovorans
?
-
-
-
?
sarcosine + O2 + H2O
about 200fold decrease in rate compared to 4-methylaminobutanoate
719401
Paenarthrobacter nicotinovorans
glycine + formaldehyde + H2O2
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 90000, SDS-PAGE, 1 * 89395, calculated
Paenarthrobacter nicotinovorans
Synonyms
Synonyms
Commentary
Organism
gamma-N-methylaminobutanoate oxidase
-
Paenarthrobacter nicotinovorans
gamma-N-methylaminobutyrate oxidase
-
Paenarthrobacter nicotinovorans
mabO
-
Paenarthrobacter nicotinovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4
-
sarcosine
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
800
-
4-methylaminobutanoate
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
10
-
Paenarthrobacter nicotinovorans
Cofactor
Cofactor
Commentary
Organism
Structure
flavin
1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor
Paenarthrobacter nicotinovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Paenarthrobacter nicotinovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
flavin
1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor
Paenarthrobacter nicotinovorans
Engineering (protein specific)
Protein Variants
Commentary
Organism
H67A
mutation results in resulted in a protein without covalently bound flavin. Mutant protein contains noncovalently bound flavin and exhibits 10% of the enzyme activity of the wild-type
Paenarthrobacter nicotinovorans
W66S
mutation results in resulted in a protein without covalently bound flavin and complete loss of actiovity
Paenarthrobacter nicotinovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
4-methylaminobutanoate
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
25
-
sarcosine
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
89395
-
1 * 90000, SDS-PAGE, 1 * 89395, calculated
Paenarthrobacter nicotinovorans
90000
-
gel filtration
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-methylaminobutanoate + O2 + H2O
-
719401
Paenarthrobacter nicotinovorans
4-aminobutanoate + formaldehyde + H2O2
-
-
-
?
additional information
no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid
719401
Paenarthrobacter nicotinovorans
?
-
-
-
?
sarcosine + O2 + H2O
about 200fold decrease in rate compared to 4-methylaminobutanoate
719401
Paenarthrobacter nicotinovorans
glycine + formaldehyde + H2O2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 90000, SDS-PAGE, 1 * 89395, calculated
Paenarthrobacter nicotinovorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4
-
sarcosine
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
800
-
4-methylaminobutanoate
pH 10, temperature not specified in the publication
Paenarthrobacter nicotinovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
10
-
Paenarthrobacter nicotinovorans
Expression
Organism
Commentary
Expression
Paenarthrobacter nicotinovorans
expression only upon growth in presence of nicotine
up
Expression (protein specific)
Organism
Commentary
Expression
Paenarthrobacter nicotinovorans
expression only upon growth in presence of nicotine
up
Other publictions for EC 1.5.3.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719443
Chiribau
Final steps in the catabolism ...
Paenarthrobacter nicotinovorans
FEBS J.
273
1528-1536
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719702
Chiribau
Characterization of PmfR, the ...
Paenarthrobacter nicotinovorans
J. Bacteriol.
187
3062-3070
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
719401
Chiribau
A novel gamma-N-methylaminobut ...
Paenarthrobacter nicotinovorans
Eur. J. Biochem.
271
4677-4684
2004
-
-
1
-
2
-
-
2
-
-
2
-
-
3
-
-
-
-
-
-
-
-
3
1
3
-
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
3
1
-
-
-
2
1
-
-
-
1
-
-
1
-
-