A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1

Chiribau, C.; Sandu, C.; Fraaije, M.; Schiltz, E.; Brandsch, R.; Eur. J. Biochem. 271, 4677-4684 (2004)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.14

-

4-methylaminobutanoate

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

25

-

sarcosine

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

4-methylaminobutanoate + O2 + H2O

-

719401

Paenarthrobacter nicotinovorans

4-aminobutanoate + formaldehyde + H2O2

-

-

-

?

additional information

no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid

719401

Paenarthrobacter nicotinovorans

?

-

-

-

?

sarcosine + O2 + H2O

about 200fold decrease in rate compared to 4-methylaminobutanoate

719401

Paenarthrobacter nicotinovorans

glycine + formaldehyde + H2O2

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

4

-

sarcosine

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

800

-

4-methylaminobutanoate

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

Cofactor

Commentary

Organism

Structure

flavin

1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor

Paenarthrobacter nicotinovorans

Cofactor

Commentary

Organism

Structure

flavin

1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor

Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.14

-

4-methylaminobutanoate

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

25

-

sarcosine

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

4-methylaminobutanoate + O2 + H2O

-

719401

Paenarthrobacter nicotinovorans

4-aminobutanoate + formaldehyde + H2O2

-

-

-

?

additional information

no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid

719401

Paenarthrobacter nicotinovorans

?

-

-

-

?

sarcosine + O2 + H2O

about 200fold decrease in rate compared to 4-methylaminobutanoate

719401

Paenarthrobacter nicotinovorans

glycine + formaldehyde + H2O2

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

4

-

sarcosine

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

800

-

4-methylaminobutanoate

pH 10, temperature not specified in the publication

Paenarthrobacter nicotinovorans

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

719443

Chiribau

Final steps in the catabolism ...

Paenarthrobacter nicotinovorans

FEBS J.

273

1528-1536

2006

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

719702

Chiribau

Characterization of PmfR, the ...

Paenarthrobacter nicotinovorans

J. Bacteriol.

187

3062-3070

2005

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

719401

Chiribau

A novel gamma-N-methylaminobut ...

Paenarthrobacter nicotinovorans

Eur. J. Biochem.

271

4677-4684

2004

-

-

1

-

2

-

-

2

-

-

2

-

-

3

-

-

-

-

-

-

-

-

3

1

3

-

-

-

2

1

-

-

1

-

-

-

-

-

1

1

-

2

-

-

-

-

2

-

-

2

-

-

-

-

-

-

-

-

-

3

1

-

-

-

2

1

-

-

-

1

-

-

1

-

-