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Literature summary for 1.5.3.18 extracted from

  • Yoshida, N.; Akazawa, S.I.; Katsuragi, T.; Tani, Y.
    Characterization of two fructosyl-amino acid oxidase homologs of Schizosaccharomyces pombe (2004), J. Biosci. Bioeng., 97, 278-280.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.02
-
L-saccharopine pH 8.0, 30°C Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
1 * 49000, SDS-PAGE Schizosaccharomyces pombe
56000
-
gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-saccharopine + H2O + O2 Schizosaccharomyces pombe Fap2 may play a role in L-lysine metabolism when a large amount of L-saccharopine is accumulated L-2-aminoadipate 6-semialdehyde + L-glutamate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9UTM9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.72
-
pH 8.0, 30°C Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-saccharopine + H2O + O2
-
Schizosaccharomyces pombe L-2-aminoadipate 6-semialdehyde + L-glutamate + H2O2
-
?
L-saccharopine + H2O + O2 Fap2 may play a role in L-lysine metabolism when a large amount of L-saccharopine is accumulated Schizosaccharomyces pombe L-2-aminoadipate 6-semialdehyde + L-glutamate + H2O2
-
?
additional information no activity with L-pipecolate, sarcosine, L-proline, Nepsilon-fructosyl Nalpha-Z-lysine or fructosyl valine Schizosaccharomyces pombe ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
FAP2
-
Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
FAD covalently linked to the protein molecule Schizosaccharomyces pombe