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Literature summary for 1.5.3.17 extracted from

  • Liu, T.; Kim, D.W.; Niitsu, M.; Berberich, T.; Kusano, T.
    Oryza sativa polyamine oxidase 1 back-converts tetraamines, spermine and thermospermine, to spermidine (2014), Plant Cell Rep., 33, 143-151 .
    View publication on PubMed
Search for more literature for 1.5.3.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 thermospermine pH 6.0, 30°C Oryza sativa
0.0049
-
 spermine pH 6.0, 30°C Oryza sativa
0.0061
-
 spermine pH 8.5, 30°C Oryza sativa
0.011
-
 norspermine pH 6.0, 30°C Oryza sativa
0.0145
-
 norspermine pH 8.5, 30°C Oryza sativa
0.0359
-
 N1-acetylspermine pH 8.5, 30°C Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Oryza sativa 5737
-

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-
Oryza sativa cv. Nipponbare
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root of seedlings Oryza sativa
-
seedling root of seedlings Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-acetylspermine + O2 + H2O
-
 Oryza sativa spermidine + 3-acetamidopropanal + H2O2
-
 ?
N1-acetylspermine + O2 + H2O
-
 Oryza sativa cv. Nipponbare spermidine + 3-acetamidopropanal + H2O2
-
 ?
norspermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa ?
-
 ?
norspermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa cv. Nipponbare ?
-
 ?
spermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa spermidine + 3-aminopropanal + H2O2
-
 ?
spermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa cv. Nipponbare spermidine + 3-aminopropanal + H2O2
-
 ?
thermospermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa ?
-
 ?
thermospermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa cv. Nipponbare ?
-
 ?

Synonyms

Synonyms Comment Organism
OsPAO1
-
 Oryza sativa
polyamine oxidase 1
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 substrate: therospermine Oryza sativa
35
-
 substrate: spermine Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
 norspermine pH 6.0, 30°C Oryza sativa
0.022
-
 N1-acetylspermine pH 8.5, 30°C Oryza sativa
0.025
-
 spermine pH 6.0, 30°C Oryza sativa
0.036
-
 norspermine pH 8.5, 30°C Oryza sativa
0.043
-
 spermine pH 8.5, 30°C Oryza sativa
0.051
-
 thermospermine pH 6.0, 30°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 substrate: thermospermine Oryza sativa
8.5
-
 spermine Oryza sativa

Expression

Organism Comment Expression
Oryza sativa expression of the enzyme OsPAO1 appears to be quite low under physiological conditions, but is markedly induced in rice roots by spermine or thermospermine treatment up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.61
-
 N1-acetylspermine pH 8.5, 30°C Oryza sativa
1.64
-
 norspermine pH 6.0, 30°C Oryza sativa
2.48
-
 norspermine pH 8.5, 30°C Oryza sativa
5.1
-
 spermine pH 6.0, 30°C Oryza sativa
6.4
-
 spermine pH 8.5, 30°C Oryza sativa
13.8
-
 thermospermine pH 6.0, 30°C Oryza sativa