Literature summary for 1.5.3.16 extracted from

Cervelli, M.; Leonetti, A.; Cervoni, L.; Ohkubo, S.; Xhani, M.; Stano, P.; Federico, R.; Polticelli, F.; Mariottini, P.; Agostinelli, E.
Stability of spermine oxidase to thermal and chemical denaturation comparison with bovine serum amine oxidase (2016), Amino Acids, 48, 2283-2291 .

Search for more literature for 1.5.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 DE3	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	a mutant enzyme deprived of all but two cysteine residues (C263/C429) results in a monomeric enzyme species. The mutant enzyme is less stable than the wild type. Is activity decreased by about 90% after 1 day from the preparation, and storage at 6°C or -20°C	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	spermine	mutant enzyme deprived of all but two cysteine residues (C263/C429), pH 8.3, 25°C	Mus musculus
0.09	-	spermine	wild-type enzyme, pH 8.3, 25°C	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q99K82	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Storage Stability

Storage Stability	Organism
-20°C, activity decreased by about 90% after 1 day, mutant enzyme deprived of all but two cysteine residues (C263/C429)	Mus musculus
6°C, activity decreased by about 90% after 1 day, mutant enzyme deprived of all but two cysteine residues (C263/C429)	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
spermine + O2 + H2O	-	Mus musculus	spermidine + 3-aminopropanal + H2O2	-	?

Subunits

Subunits	Comment	Organism
dimer	homodimeric and monomeric enzyme forms are catalytically active	Mus musculus
monomer	homodimeric and monomeric enzyme forms are catalytically active. A mutant enzyme deprived of all but two cysteine residues (C263/C429) results in a monomeric enzyme species	Mus musculus

Synonyms

Synonyms	Comment	Organism
SMOX	-	Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
62.8	-	Tm-value	Mus musculus
100	-	thermal denaturation is irreversible after heating to 100°C	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.32	-	spermine	mutant enzyme deprived of all but two cysteine residues (C263/C429), pH 8.3, 25°C	Mus musculus
3.5	-	spermine	wild-type enzyme, pH 8.3, 25°C	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
16	-	spermine	mutant enzyme deprived of all but two cysteine residues (C263/C429), pH 8.3, 25°C	Mus musculus
50	-	spermine	wild-type enzyme, pH 8.3, 25°C	Mus musculus

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Release 2023.1 (January 2023)