Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.3.16 extracted from

  • Bellelli, A.; Cavallo, S.; Nicolini, L.; Cervelli, M.; Bianchi, M.; Mariottini, P.; Zelli, M.; Federico, R.
    Mouse spermine oxidase: a model of the catalytic cycle and its inhibition by N,N1-bis(2,3-butadienyl)-1,4-butanediamine (2004), Biochem. Biophys. Res. Commun., 322, 1-8.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 DE3 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
N,N1-bis(2,3-butadienyl)-1,4-butanediamine i.e. MDL72527, competitive. If incubated for longer times, MDL72527 yields irreversible inhibition of the enzyme with a half-life of 15 min at 0.1 mM MDL72527 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
spermine
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
x * 63000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q99K82
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with spermidine and putrescine Mus musculus ?
-
?
spermine + O2 + H2O the mMSO catalytic mechanism is consistent with a simple four-step kinetic scheme. The enzyme is unable to oxidize other free or acetylated polyamines Mus musculus ?
-
?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
mSMO
-
Mus musculus
spermine oxidase
-
Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8
-
spermine
-
Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
FAD non-covalently bound to the enzyme Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.063
-
N,N1-bis(2,3-butadienyl)-1,4-butanediamine
-
Mus musculus