Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.3.13 extracted from

  • Wang, H.; Liu, B.; Li, H.; Zhang, S.
    Identification and biochemical characterization of polyamine oxidases in amphioxus Implications for emergence of vertebrate-specific spermine and acetylpolyamine oxidases (2016), Gene, 575, 429-437.
    View publication on PubMed
Search for more literature for 1.5.3.13

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes expressed in Escherichia coli Branchiostoma japonicum

Protein Variants

Protein Variants Comment Organism
H69E compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
H69Q compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
K315M compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
T467S compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
T467Y compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum

Inhibitors

Inhibitors Comment Organism Structure
N1,N4-bis(2,3-butadienyl)-1,4-butanediamine 2.5 mM, 70% inhibition of N1-acetylspermine oxidation Branchiostoma japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0369
-
 N1-acetylspermine 37°C, pH 8.0, wild-type enzyme Branchiostoma japonicum
0.043
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme K315M Branchiostoma japonicum
0.046
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467Y Branchiostoma japonicum
0.058
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467S Branchiostoma japonicum
0.062
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69E Branchiostoma japonicum
0.071
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69Q Branchiostoma japonicum

Organism

Organism UniProt Comment Textmining
Branchiostoma japonicum A0A0N7IZL9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Branchiostoma japonicum

Source Tissue

Source Tissue Comment Organism Textmining
gill
-
 Branchiostoma japonicum
-
hepatic cecum
-
 Branchiostoma japonicum
-
hindgut
-
 Branchiostoma japonicum
-
muscle
-
 Branchiostoma japonicum
-
notochord
-
 Branchiostoma japonicum
-
ovary
-
 Branchiostoma japonicum
-
testis
-
 Branchiostoma japonicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Bjpao2 shows no activity with spermine Branchiostoma japonicum ?
-
 ?
N1-acetylspermine + O2 + H2O
-
 Branchiostoma japonicum spermidine + 3-acetamidopropanal + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Branchiostoma japonicum

Synonyms

Synonyms Comment Organism
Bjpao2
-
 Branchiostoma japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Branchiostoma japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate: N1-acetylspermine Branchiostoma japonicum

pI Value

Organism Comment pI Value Maximum pI Value
Branchiostoma japonicum calculated from sequence
-
 5.13