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Literature summary for 1.5.3.10 extracted from

  • Casaite, V.; Poviloniene, S.; Meskiene, R.; Rutkiene, R.; Meskys, R.
    Studies of dimethylglycine oxidase isoenzymes in Arthrobacter globiformis cells (2011), Curr. Microbiol., 62, 1267-1273.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glycine betaine
-
Arthrobacter globiformis

Cloned(Commentary)

Cloned (Comment) Organism
two dmg genes, dmg and dmg2, DNA and amino acid sequence determination, genetic organization, quantitative real-time pCR expression analysis Arthrobacter globiformis

Inhibitors

Inhibitors Comment Organism Structure
NaCl the specific activity of DMGO decreased when cells are grown in the medium with high NaCl concentration. High osmolarity inhibits the activity of the enzyme, but not in the case of the medium with glycine betaine as the sole carbon source Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.39
-
N,N-dimethylglycine DMGO1, pH 9.0, temperature not specified in the publication Arthrobacter globiformis
1.86
-
N,N-dimethylglycine DMGO2, pH 9.0, temperature not specified in the publication Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N-dimethylglycine + H2O + O2 Arthrobacter globiformis
-
sarcosine + formaldehyde + H2O2
-
?
N,N-dimethylglycine + H2O + O2 Arthrobacter globiformis NRRL B-2979
-
sarcosine + formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
two dmg genes, dmg and dmg2
-
Arthrobacter globiformis NRRL B-2979
-
two dmg genes, dmg and dmg2
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:glycine betaine-grown cell
-
Arthrobacter globiformis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylglycine + H2O + O2
-
Arthrobacter globiformis sarcosine + formaldehyde + H2O2
-
?
N,N-dimethylglycine + H2O + O2
-
Arthrobacter globiformis NRRL B-2979 sarcosine + formaldehyde + H2O2
-
?

Synonyms

Synonyms Comment Organism
dimethylglycine dehydrogenase
-
Arthrobacter globiformis
DMGO
-
Arthrobacter globiformis
DMGO1
-
Arthrobacter globiformis
DMGO2
-
Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
DMGO2 Arthrobacter globiformis
37
-
DMGO1 Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
DMGO1 and DMGO2 Arthrobacter globiformis

Expression

Organism Comment Expression
Arthrobacter globiformis glycine betaine induces the expression of the dmg genes by about 300fold at 5 mM up

General Information

General Information Comment Organism
metabolism in high-salinity media with 1 M NaCl, Arthrobacter globiformis accumulates 360 mM of glycine betaine when the main source of carbon is glucose and 180 mM glycine betaine when the sole carbon source is glycine betaine Arthrobacter globiformis