Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycine betaine | - |
Arthrobacter globiformis |
Cloned (Comment) | Organism |
---|---|
two dmg genes, dmg and dmg2, DNA and amino acid sequence determination, genetic organization, quantitative real-time pCR expression analysis | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | the specific activity of DMGO decreased when cells are grown in the medium with high NaCl concentration. High osmolarity inhibits the activity of the enzyme, but not in the case of the medium with glycine betaine as the sole carbon source | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.39 | - |
N,N-dimethylglycine | DMGO1, pH 9.0, temperature not specified in the publication | Arthrobacter globiformis | |
1.86 | - |
N,N-dimethylglycine | DMGO2, pH 9.0, temperature not specified in the publication | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + H2O + O2 | Arthrobacter globiformis | - |
sarcosine + formaldehyde + H2O2 | - |
? | |
N,N-dimethylglycine + H2O + O2 | Arthrobacter globiformis NRRL B-2979 | - |
sarcosine + formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
two dmg genes, dmg and dmg2 | - |
Arthrobacter globiformis NRRL B-2979 | - |
two dmg genes, dmg and dmg2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:glycine betaine-grown cell | - |
Arthrobacter globiformis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + H2O + O2 | - |
Arthrobacter globiformis | sarcosine + formaldehyde + H2O2 | - |
? | |
N,N-dimethylglycine + H2O + O2 | - |
Arthrobacter globiformis NRRL B-2979 | sarcosine + formaldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dimethylglycine dehydrogenase | - |
Arthrobacter globiformis |
DMGO | - |
Arthrobacter globiformis |
DMGO1 | - |
Arthrobacter globiformis |
DMGO2 | - |
Arthrobacter globiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
DMGO2 | Arthrobacter globiformis |
37 | - |
DMGO1 | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
DMGO1 and DMGO2 | Arthrobacter globiformis |
Organism | Comment | Expression |
---|---|---|
Arthrobacter globiformis | glycine betaine induces the expression of the dmg genes by about 300fold at 5 mM | up |
General Information | Comment | Organism |
---|---|---|
metabolism | in high-salinity media with 1 M NaCl, Arthrobacter globiformis accumulates 360 mM of glycine betaine when the main source of carbon is glucose and 180 mM glycine betaine when the sole carbon source is glycine betaine | Arthrobacter globiformis |